Recent advances in centrifugal microfluidic chip-based loop-mediated isothermal amplification

文献类型： 外文期刊

第一作者： Xiao, Bin

作者： Xiao, Bin;Zhao, Ruiming;Wang, Nan;Zhang, Juan;Sun, Xiaoyun;Chen, Ailiang

作者机构：

关键词： Loop -mediated isothermal amplification; Centrifugal microfluidic chips; Chip construction; DNA extraction; Fluid control; Signal production; Portable device; Point-of-care

期刊名称：TRAC-TRENDS IN ANALYTICAL CHEMISTRY （ 影响因子：13.1； 五年影响因子：13.1 ）

ISSN： 0165-9936

年卷期： 2023 年 158 卷

页码：

收录情况： SCI

摘要： Loop-mediated isothermal amplification (LAMP) is a thermostatic nucleic acid amplification technology that is widely used in the fields of clinical diagnosis and food safety detection. Centrifugal microfluidic chips are developed based on traditional microfluidic chips, using centrifugal force to achieve liquid flowing, mixing and reaction, eliminating the design of complex valves and pumps. The combination of LAMP and centrifugal microfluidic chips has great potential for rapid field nucleic acid detection. In this review, we discuss the design of a centrifugal microfluidic chip based on LAMP technology, including the chip fabrication, DNA extraction chamber, fluid control technology, common dye types in the reaction chamber, and the supporting detection device. It aims to provide some ideas and references for the further development and application of centrifugal microfluidic chip-based loop-mediated isothermal amplification in fast, on-site nucleic acid detection such as point-of-care tests and food safety. & COPY; 2022 Elsevier B.V. All rights reserved.

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