Characterization of three new S-alleles and development of an S-allele-specific PCR system for rapidly identifying the S-genotype in apple cultivars
文献类型: 外文期刊
第一作者: Long, Shenshan
作者: Long, Shenshan;Li, Maofu;Han, Zhenhai;Li, Tianzhong;Wang, Kun
作者机构:
关键词: malus domestica borkh;apple;self-incompatibility;S-alleles;PCR
期刊名称:TREE GENETICS & GENOMES ( 影响因子:2.297; 五年影响因子:2.547 )
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收录情况: SCI
摘要: Apple (Malus domestica Borkh), a member of the Rosaceae, shows gametophytic self-incompatibility (GSI) controlled by polymorphic S-alleles. Identifying the S-genotypes of apple cultivars can be applied on correct assignment of apple cultivars to cross-compatibility groups, which is important for the efficient production of apple fruit. This study characterized three new S-alleles (designated S_(44), S_(45), and S_(46)) in apple and developed an efficient analysis method that can be used to characterize S-genotypes by utilizing allele-specific polymerase chain reaction rapidly. Nineteen allele-specific primers were selectively designed to identify different alleles. Using this method, S-genotypes of 157 apple cultivars were identified.
分类号: NULL
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