Molecular characterization and immune protection by cystathionine beta-synthase from Eimeria tenella

文献类型： 外文期刊

第一作者： Zhao, Huanzhi

作者： Zhao, Huanzhi;Li, Cong;Zhu, Shunhai;Zhao, Qiping;Dong, Hui;Huang, Bing;Han, Hongyu

作者机构：

关键词： characterization; chicken coccidiosis; cystathioninc beta-synthase; Eimeria tenella; vaccine

期刊名称：JOURNAL OF EUKARYOTIC MICROBIOLOGY （ 影响因子：3.88； 五年影响因子：2.867 ）

ISSN： 1066-5234

年卷期： 2022 年 69 卷 2 期

页码：

收录情况： SCI

摘要： Eimeria tenella is an obligate intracellular apicomplexan parasite that causes avian coccidiosis and leads to severe economic losses in the global poultry industry. Cystathionine beta-synthase (CBS) and cystathionine gamma-lyase (CGL) act together to generate H2S in the reverse transsulfuration pathway. In this study, E. tenella Cystathionine beta-synthase (EtCBS) was cloned using rapid amplification of cDNA 5'-ends (5'RACE) and characterized, and its immunoprotective effects were evaluated. The recombinant EtCBS protein (rEtCBS) was expressed and successfully recognized by anti-sporozoites (Spz) protein rabbit serum. EtCBS mRNA levels were highest in Spz by qPCR, and the protein expression levels were higher in unsporulated oocysts (UO) than in other stages by Western blot. Indirect immunofluorescence showed that EtCBS protein was found on the surface of Spz and second-generation merozoites (Mrz). The invasion inhibition assays showed that rabbit anti-rEtCBS polyclonal antibodies effectively inhibited parasite invasion host cells. Chickens immunized with rEtCBS protein showed prominently increased weight gains and decreased oocyst output compared to nonimmunized and infected control group. The results suggest that EtCBS could be a potential vaccine candidate against E. tenella.

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