Massilia luteola sp. nov., a novel indole- producing and cellulosedegrading bacterium isolated from soil

文献类型： 外文期刊

第一作者： Huang, Xinbing

作者： Huang, Xinbing;Qi, Shengtao;Song, Wenshuai;Yu, Xiaoxin;Zhang, Haifeng;Xiang, Wensheng;Zhao, Junwei;Wang, Xiangjing;Xiang, Wensheng

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关键词： 16S rRNA gene; cellulose- degrading; genome characterization; indole- producing; Massilia luteola sp. nov.

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.8； 五年影响因子：2.6 ）

ISSN： 1466-5026

年卷期： 2024 年 74 卷 4 期

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收录情况： SCI

摘要： A Gram- stain- negative, rod- shaped, indole- producing, and cellulosedegrading bacterial strain, designated NEAU- G- C5 T , was isolated from soil collected from a forest in Dali city, Yunnan province, south China. 16S rRNA gene sequence analysis showed that strain NEAU-G-C5 T was assigned to the genus Massilia and showed high sequence similarities to Massilia phosphatilytica 12-OD1 T (98.32 %) and Massilia putida 6 NM -7 T (98.41 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-G-C5 T formed a lineage related to M. phosphatilytica 12-OD1 T and M. putida 6 NM -7 T . The major fatty acids of the strain were C 16:0 , C 16: 1 omega 7 c , and C 17:0 cyclo. The respiratory quinone was Q- 8. The polar lipid profile of the strain showed the presence of diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. In addition, the average nucleotide identity values between strain NEAU- G- C5 T and its reference strains M. phosphatilytica 12-OD1 T , M. putida 6 NM -7 T , M. norwichensis NS9 T , and M. kyonggiensis TSA1 T were 89.7, 88.2, 81.3, and 88.0 %, respectively, and the levels of digital DNA-DNA hybridization between them were found to be 58.5 % (54.9-62.0 %), 53.2 % (49.8-56.7 %), 31.9 % (28.6-35.5 %), and 57.7 % (54.1-61.2 %), respectively, which were lower than the accepted threshold values of 95-96 % and 70 %, respectively. The DNA G+C content of strain NEAU-G-C5 T was 66.5 mol%. The strain could produce indoleacetic acid and cellulase. On the basis of the phenotypic, genotypic, and chemotaxonomic characteristics, we conclude that strain NEAU- G- C5 T represents a novel species of the genus Massilia , for which the name Massilia luteola sp. nov. is proposed. The type strain is NEAU- G- C5 T (=MCCC 1K08668 T =KCTC 8080 T ).

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