Identification and evaluation of an endophytic antagonistic yeast for the control of gray mold (Botrytis cinerea) in apple and mechanisms of action

文献类型： 外文期刊

第一作者： Yu, Xiaoyan

作者： Yu, Xiaoyan;Zhang, Kuan;Liu, Jiayi;Zhao, Zhenhua;Guo, Bowen;Wang, Xiangjing;Xiang, Wensheng;Zhao, Junwei;Xiang, Wensheng

作者机构：

关键词： Apple gray mold; Botrytis cinerea; Bullera alba PGY-2; Biocontrol efficacy; Defense -related enzyme

期刊名称：FOOD MICROBIOLOGY （ 影响因子：4.5； 五年影响因子：5.1 ）

ISSN： 0740-0020

年卷期： 2024 年 123 卷

页码：

收录情况： SCI

摘要： Gray mold, caused by Botrytis cinerea , is a prevalent postharvest disease of apple that limits their shelf life, resulting in significant economic losses. The use of antagonistic microorganisms has been shown to be an effective approach for managing postharvest diseases of fruit. In the present study, an endophytic yeast strain PGY-2 was isolated from apples and evaluated for its biocontrol efficacy against gray mold and its mechanisms of action. Results indicated that strain PGY-2, identified as Bullera alba , reduced the occurrence of gray mold on apples and significantly inhibited lesion development in pathogen -inoculated wounds. Gray mold control increased with the use of increasing concentrations of PGY-2, with the best disease control observed at 10 8 cells/ mL. Notably, Bullera alba PGY-2 did not inhibit the growth of Botrytis cinerea in vitro indicating that the yeast antagonist did not produce antimicrobial compounds. The rapid colonization and stable population of PGY-2 in apple wounds at 4 degrees C and 25 degrees C confirmed its ability to compete with pathogens for nutrients and space. PGY-2 also had a strong ability to form a biofilm and enhanced the activity of multiple defense -related enzymes (POD, PPO, APX, SOD, PAL) in host tissues. Our study is the first time to report the use of Bullera alba PGY-2 as a biocontrol agent for postharvest diseases of apple and provide evidence that Bullera alba PGY-2 represents an endophytic antagonistic yeast with promising biocontrol potential and alternative to the use of synthetic, chemical fungicides for the control of postharvest gray mold in apples.

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