Multiple Metabolic Enzymes Can Be Involved in Cross-Resistance to 4-Hydroxyphenylpyruvate-Dioxygenase-Inhibiting Herbicides in Wild Radish

文献类型： 外文期刊

第一作者： Lu, Huan

作者： Lu, Huan;Liu, Yingze;Li, Mengshuo;Qiang, Sheng;Lu, Huan;Han, Heping;Yu, Qin;Powles, Stephen;Zhou, Fengyan;Nyporko, Alex

作者机构：

关键词： RNA sequencing; Rr2ODD1; herbicide metabolic resistance

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年 71 卷 24 期

页码：

收录情况： SCI

摘要： A wild radish population (R) has been recently confirmedto becross-resistant to 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibitingherbicides without previous exposure to these herbicides. This cross-resistanceis endowed by enhanced metabolism. Our study identified one 2-oxoglutarate/Fe-(II)-dependentdioxygenase gene (Rr2ODD1) and two P450 genes (RrCYP704C1 and RrCYP709B1), which weresignificantly more highly expressed in R versus susceptible (S) plants.Gene functional characterization using Arabidopsis transformation showed that overexpression of RrCYP709B1 conferred a modest level of resistance to mesotrione. Ultra-performanceliquid chromatography-tandem mass spectrometry analysis showedthat tissue mesotrione levels in RrCYP709B1 transgenic Arabidopsis plants were significantly lower thanthat in the wild type. In addition, overexpression of Rr2ODD1 or RrCYP704C1 in Arabidopsis endowed resistance to tembotrione and isoxaflutole. Structural modelingindicated that mesotrione can bind to CYP709B1 and be easily hydroxylatedto form 4-OH-mesotrione. Although each gene confers a modest levelof resistance, overexpression of the multiple herbicide-metabolizinggenes could contribute to HPPD-inhibiting herbicide resistance inthis wild radish population.

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