Glyphosate Resistance in Eleusine indica: Involvement of CYP71AK44 in Addition to EPSPS Gene Overexpression

文献类型： 外文期刊

第一作者： He, Sifen

作者： He, Sifen;Bai, Lianyang;Tian, Junhui;Ouyang, Yulan;Liao, Yuxi;Bai, Lianyang;Pan, Lang;Bai, Lianyang;Yu, Qin

作者机构：

关键词： non-target-site resistance (NTSR); EPSPS overexpression; CYP71AK44

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2024 年 72 卷 43 期

页码：

收录情况： SCI

摘要： Intensive application of glyphosate has resulted in resistance evolution in many weed populations, including Eleusine indica. This study characterized glyphosate resistance and investigated the underlying mechanisms in a glyphosate-resistant population (R-JX) of E. indica from China. The R-JX population was 8.5 times resistant to glyphosate relative to the glyphosate-susceptible population (SA). Point mutations were not observed in the target gene 5-enolypyruvyl-shikimate-3-phosphate synthase gene (EPSPS). However, the expression level and copy number of EPSPS were 8.8 times and 15.2 times, respectively, greater in R-JX than that in the SA population. Pre-application of the P450 inhibitor lowered the resistance level to glyphosate from 8.5 times to 3.6 times in the R-JX population. RNA-Seq and RT-qPCR revealed that the CYP71AK44 gene was consistently upregulated in R-JX and five other glyphosate-resistant populations. Rice calli and seedlings overexpressing CYP71AK44 showed glyphosate resistance. In conclusion, overexpression of the target EPSPS plus CYP71AK44 collectively contributes to glyphosate resistance in these E. indica populations.

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