Technology for Distribution and Control of Agrobacterium tumefaciens in Cherry Tree Soil

文献类型: 外文期刊

第一作者: Liu, Shenyan

作者: Liu, Shenyan;Han, Qingli;Liu, Shenyan;Gao, Shimeng;Cao, Aocheng;Zheng, Yiwen;Yu, Kunpeng;Zhao, Xiaojuan

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关键词: crown gall; cherry tree; Agrobacterium tumefaciens; qPCR detection; soil fumigation; control efficiency; soil pathogen management

期刊名称:AGRICULTURE-BASEL ( 影响因子:3.6; 五年影响因子:3.8 )

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年卷期: 2024 年 14 卷 11 期

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收录情况: SCI

摘要: Sakura crown gall, caused by the invasion of Agrobacterium tumefaciens through plant wounds, poses a significant threat to cherry trees. In this study, the distribution of A. tumefaciens was preliminarily determined by stratified sampling and qPCR detection. Vertically, the pathogen is mainly distributed in the soil layer below 20 cm, and the amount of bacteria increases at greater depths. Horizontally, they are found within a 150 cm radius from the trunk. Zhongshengmycin and Oligosaccharide-Ethylicin were applied 100 cm from the trunk at a depth of below 20 cm. In the 20 similar to 40 cm soil layer, a 600-fold diluted solution of 3% Zhongshengmycin had a relative control efficacy of 94% to 100% against Agrobacterium tumefaciens, while a 1000-fold diluted solution of 25% Oligosaccharide-Ethylicin showed a control effect ranging from 54% to 100%. Before transplantation, the soil was disinfected with dazomet and abamectin. Application rates were 35 g/m(2) for dazomet (98% granules) and 1 mL/m(2) for abamectin (1.8% emulsifiable concentrate). The disinfection effectiveness was 77 similar to 100% in the 0 similar to 60 cm soil layer.

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