Stable expression of foot-and-mouth disease virus protein VP1 fused with cholera toxin B subunit in the potato (Solanum tuberosum)

文献类型： 外文期刊

第一作者： He, Dong-Mei

作者： He, Dong-Mei;Qian, Kai-Xian;Shen, Gui-Fang;Li, Yi-Na;Zhang, Zhi-Fang;Su, Zhong-Liang;Shao, Hong-Bo

作者机构：

关键词： cholera toxin B subunit (CTB);foot-and-mouth disease virus (FMDV);fused gene;immunogenicity;patatin promoter;fused proterin;gene engineering;protein fusion;TRANSGENIC PLANTS;ANTIBODY-RESPONSE;MUCOSAL VACCINES;VACCINATION;INDUCTION;STORAGE;TOBACCO

期刊名称：COLLOIDS AND SURFACES B-BIOINTERFACES （ 影响因子：5.268； 五年影响因子：4.957 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： The expression vector, pB1121CTBVP1, containing the fusion of the foot and Mouth disease virus (FMDV) VP1 gene and the cholera toxin B subunit (CTB) gene was constructed by fused PCR and transferred into potato (Solanum tuberosum L.) by Agrobacterium-mediated transformation. Transformed plants were obtained by selecting on kanamycin-resistant medium strictly and regenerated. The transgenic plantlets were identified by PCR, Southern-blot and the production of fused protein was confirmed and quantified by Western-blot and ELISA assays. The results showed that the fused genes were expressed stablely under the control of specific-tuber patatin promoter. The expressed fused proteins have a certain degree of immunogenicity. (C) 2006 Elsevier B.V. All rights reserved.

分类号： O6

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