A CRISPR-Cas12a-based platform facilitates the detection and serotyping of Streptococcus suis serotype 2

文献类型： 外文期刊

第一作者： Wang, Lu

作者： Wang, Lu;Sun, Jing;Zhao, Jiyu;Bai, Jieyu;Zhang, Yueling;Zhu, Yao;Zhang, Wanjiang;Wang, Chunlai;Liu, Siguo;Li, Gang;Wang, Lu;Bai, Jieyu;Zhao, Jiyu;Langford, Paul R.

作者机构：

关键词： CRISPR; Cas12a; crRNA; Streptococcus suis serotype 2; Diagnostics

期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.4 ）

ISSN： 0039-9140

年卷期： 2024 年 267 卷

页码：

收录情况： SCI

摘要： Streptococcus suis serotype 2 is an economically important zoonotic pathogen that causes septicemia, arthritis, and meningitis in pigs and humans. S. suis serotype 2 is responsible for substantial economic losses to the swine industry and poses a serious threat to public health, and accurate and rapid detection is important for the prevention and control of epidemic disease. In this study, we developed a high-fidelity detection and serotyping platform for S. suis serotype 2 based on recombinase polymerase amplification (RPA) and a clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a system called Cards-SSJ/K. Cards-SSJ had a detection limit of 10 CFU, takes <60 min, and no cross-reaction was found with other S. suis serotypes, closely related Streptococcus spp., or common pig pathogens, and Cards-SSK could differentiate serotype 2 from serotype 1/2. Results from Cards-SSJ and qPCR were equivalent in detecting S. suis serotype 2 in tissue samples. Analysis indicated that despite a relatively high reagent cost compared to PCR and qPCR, Cards-SSJ was less timeconsuming and had low requirements for equipment and personnel. Thus, it is an excellent method for pointof-care detection for S. suis serotype 2.

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