UvWhi2 Is Required for Stress Response and Pathogenicity in Ustilaginoidea virens

文献类型： 外文期刊

第一作者： Meng Shuai

作者： Meng Shuai;Qiu Jiehua;Xiong Meng;Liu Zhiquan;Jagernath, Jane Sadhna;Shi Huanbin;Kou Yanjun;Meng Shuai;Lin Fucheng;Lin Fucheng

作者机构：

关键词： Whi2; rice false smut; Ustilaginoidea virens; pathogenesis; secondary spore

期刊名称：RICE SCIENCE （ 影响因子：4.412； 五年影响因子：5.026 ）

ISSN： 1672-6308

年卷期： 2022 年 29 卷 1 期

页码：

收录情况： SCI

摘要： Some stress response-related genes have been identified in Ustilaginoidea virens, but it is not clear whether and how defects of stress responses affect the pathogenesis processes of U. virens. In this study, we identified a general stress response factor UvWHI2 as a homolog of Saccharomyces cerevisiae Whi2 in U. virens. The relative expression level of UvWhi2 was significantly up-regulated during infection, suggesting that UvWHI2 may be involved in pathogenesis. Furthermore, knockout of UvWhi2 showed decreased mycelial growth, increased conidiation in the potato sucrose medium and a defect in pathogenicity. In addition, the RNA-Seq and phenotypic analysis showed that UvWHI2 was involved in response to oxidative, hyperosmotic, cell wall stress and nutrient limitation. Further studies revealed that the defects of stress responses of the Delta Uvwhi2 mutant affected the formation of secondary spores on the nutrient limited surface and the rice surface, resulting in a significant reduction of pathogenicity of U. virens. Our results suggest that UvWHI2 is necessary for fungal growth, stress responses and the formation of secondary spores in U. virens. In addition, the defects of stress responses can affect the formation of secondary spores on the rice surface, and then compromise the pathogenicity of U. virens.

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