Antigenicity, epitope mapping, and intracellular distribution of the NSP7α protein of porcine reproductive and respiratory syndrome virus

文献类型： 外文期刊

第一作者： Wang, Tao

作者： Wang, Tao;Xia, Da-Song;Tian, Xiao-Xiao;Yang, Yong-Bo;An, Tong-Qing;An, Tong-Qing;An, Tong-Qing

作者机构：

关键词： PRRSV; NSP7 alpha; Antigenicity; Epitope; Intracellular distribution

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2024 年 265 卷

页码：

收录情况： SCI

摘要： Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen that causes huge economic losses to the global pig industry. Nonstructural protein 7 alpha (NSP7 alpha) of PRRSV is highly conserved among different lineages of PRRSV and could be a potential target for the development of detection methods. In this study, NSP7 alpha was expressed in prokaryote (Escherichia coli) and purified. An NSP7 alpha-ab-ELISA detection method was established, the NSP7 alpha-ab-ELISA has 93.1 % coincidence rate with IDEXX PRRS X3 ab test kit. NSP7 alpha antibody was detected in pig serum by ELISA 14 days following PRRSV infection. Three monoclonal antibodies (4H9, 3F2, and C10) against NSP7 alpha prepared by a hybridoma technique were used for epitope mapping by indirect immunofluorescence. The 4H9, 3F2, and C10 antibodies all recognized the C -terminal 72-149 amino acid region of NSP7 alpha. 4H9 reacted with amino acids 135-143, but 3F2 and C10 did not react with any truncated polypeptide. In addition, by using the monoclonal antibodies, NSP7 alpha was localized solely in the cytoplasm, while the N protein was distributed in the cytoplasm and nucleus. The collective findings of the antigenicity and epitope of NSP7 alpha will be helpful for understanding the antigenicity of NSP7 alpha and developing PRRSV diagnostic methods.

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