Super signal-enhancement biosensing platform for precise target recognition based on rolling circle-hybridization chain dual linear cascade amplification technology

文献类型： 外文期刊

第一作者： Tang, Danyao

作者： Tang, Danyao;Xiong, Chunyuan;Wu, Yeyu;Luo, Hu;Yan, Jun;Huang, Ke-Jing;Tan, Xuecai;Ya, Yu

作者机构：

关键词： Double-layer linear cascade amplification technology; Precise target identification; Super signal amplification; Efficient conversion

期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.5 ）

ISSN： 0039-9140

年卷期： 2025 年 285 卷

页码：

收录情况： SCI

摘要： This paper presents a self-powered biosensing platform based on graphdiyne@Au (2D GDY@Au) nanoparticles and rolling circle-hybridization chain (RC-HC) dual linear cascade amplification technology, which significantly enhances target recognition and signal amplification efficiency for miRNA-141. Specifically, the target on bioanode outputs a large amount of single-stranded DNA (T1) through the strand displacement amplification (SDA) mechanism. This efficient target recycling process triggers RC-HC dual linear cascade reaction. The RCA product and H2 form the L-Liner/H2 hybridized chain through a hybridization chain reaction, and then are immobilized on a flexible electrode using a Y-DNA capture handle. [Ru(NH3)(6)](3+) is precisely anchored in the grooves of the DNA double helix. The 2D GDY@Au enhances the electron mobility of the system to form a rich electron-donating center. The [Ru(NH3)(6)](3+) on the biocathode receives electrons and is reduced to [Ru(NH3)(6)](2+), producing a significantly amplified open-circuit voltage signal. Dual linear cascade amplification technology realizes precise target recognition, exponential amplification, and efficient conversion of biological signals. This technique displays an extensive linear range (0.0001-10000 pM) with a detection limit of 25.9 aM (S/N = 3), and it provides an innovative method for developing sensors based on nucleic acid amplification and presents a promising novel approach for the sensitive and precise detection of low-abundance target molecules, highlighting a new tactic for the creation of compact and portable analytical devices.

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