Alteration of the DNA methylation status of donor cells impairs the developmental competence of porcine cloned embryos

文献类型： 外文期刊

第一作者： Huan, Yan Jun

作者： Huan, Yan Jun;Zhu, Jiang;Xie, Bing Teng;Wang, Jian Yu;Li, Jing Yu;Xue, Bing Hua;Kong, Qing Ran;Liu, Zhong Hua;Huan, Yan Jun;Wu, Zhan Feng;Zhang, Ji Guang

作者机构：

关键词： DNA methylation; Donor cell; Nuclear reprogramming; Somatic cell nuclear transfer; Pig

期刊名称：JOURNAL OF REPRODUCTION AND DEVELOPMENT （ 影响因子：2.214； 五年影响因子：2.221 ）

ISSN： 0916-8818

年卷期： 2016 年 62 卷 1 期

页码：

收录情况： SCI

摘要： Nuclear reprogramming induced by somatic cell nuclear transfer is an inefficient process, and donor cell DNA methylation status is thought to be a major factor affecting cloning efficiency. Here, the role of donor cell DNA methylation status regulated by 5-aza-2'-deoxycytidine (5-aza-dC) or 5-methyl-2'-deoxycytidine-5'-triphosphate (5-methyl-dCTP) in the early development of porcine cloned embryos was investigated. Our results showed that 5-aza-dC or 5-methyl-dCTP significantly reduced or increased the global methylation levels and altered the methylation and expression levels of key genes in donor cells. However, the development of cloned embryos derived from these cells was reduced. Furthermore, disrupted pseudo-pronucleus formation and transcripts of early embryo development-related genes were observed in cloned embryos derived from these cells. In conclusion, our results demonstrated that alteration of the DNA methylation status of donor cells by 5-aza-dC or 5-methyl-dCTP disrupted nuclear reprogramming and impaired the developmental competence of porcine cloned embryos.

分类号： S814

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