Cloning and sequence analysis of putative type II fatty acid synthase genes from Arachis hypogaea L.

文献类型： 外文期刊

第一作者： Li, Meng-Jun

作者： Li, Meng-Jun;Li, Ai-Qin;Xia, Han;Zhao, Chuan-Zhi;Li, Chang-Sheng;Wan, Shu-Bo;Bi, Yu-Ping;Wang, Xing-Jun

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关键词： Arachis hypogaea L.; EST sequencing; gene cloning; type II FAS

期刊名称：JOURNAL OF BIOSCIENCES （ 影响因子：1.826； 五年影响因子：2.107 ）

ISSN： 0250-5991

年卷期： 2009 年 34 卷 2 期

页码：

收录情况： SCI

摘要： The cultivated peanut is a valuable source of dietary oil and ranks fifth among the world oil crops. Plant fatty acid biosynthesis is catalysed by type II fatty acid synthase (FAS) in plastids and mitochondria. By constructing a full-length cDNA library derived from immature peanut seeds and homology-based cloning, candidate genes of acyl carrier protein (ACP), malonyl-CoA:ACP transacylase, beta-ketoacyl-ACP synthase (I, II, III), beta-ketoacyl-ACP reductase, beta-hydroxyacyl-ACP dehydrase and enoyl-ACP reductase were isolated. Sequence alignments revealed that primary structures of type II FAS enzymes were highly conserved in higher plants and the catalytic residues were strictly conserved in Escherichia coli and higher plants. Homologue numbers of each type II FAS gene expressing in developing peanut seeds varied from 1 in KASII, KASIII and HD to 5 in ENR. The number of single-nucleotide polymorphisms (SNPs) was quite different in each gene. Peanut type II FAS genes were predicted to target plastids except ACP2 and ACP3. The results suggested that peanut may contain two type II FAS systems in plastids and mitochondria. The type II FAS enzymes in higher plants may have similar functions as those in E. coli.

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