UHPLC-MS/MS analysis of the influence of paclobutrazol application on Ophiopogon japonicus

文献类型： 外文期刊

第一作者： Zhao, Haoran

作者： Zhao, Haoran;Zhang, Zezhou;Xiao, Ouli;Chen, Jieyin;Dai, Xiaofeng;Kong, Zhiqiang;Chen, Jieyin;Li, Minmin;Zhao, Haoran;Wang, Ran

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关键词： Growth regulators; Ophiopogon japonicus; Saponins; Flavonoids

期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.6； 五年影响因子：4.6 ）

ISSN： 0889-1575

年卷期： 2025 年 137 卷

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收录情况： SCI

摘要： The pharmacological effects of Ophiopogon japonicus are influenced by its active substances like saponins and flavonoids, which are crucial for assessing its quality. An ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometry (UHPLC-MS/MS) method was developed for the qualitative and quantitative assessment of 20 saponins and flavonoids in O. japonicus. The average recovery of these compounds ranged from 70 % to 130 %, with relative standard deviations (RSD) between 0.11 % and 16.46 %. Planting O. japonicus in Santai County (Sichuan, China) and investigated the impact of 0.6, 1.3, 2.8, 5.6, and 11.3 g/L concentrations of paclobutrazol on both root yield and the levels of saponins and flavonoids in O. japonicus. Lower paclobutrazol concentrations (1.3 g/L) led to increased levels of saponins and flavonoids in O. japonicus such as ophiopogonin D ', ruscogenin and ophiopogonanone F, with paclobutrazol residues measuring 77.17 mu g/kg DW. This preliminary insight into the influence of paclobutrazol and production of saponin flavonoids in O. japonicus bears significance for its larger industrial production.

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