A design optimized prime editor with expanded scope and capability in plants

文献类型： 外文期刊

第一作者： Xu, Wen

作者： Xu, Wen;Yang, Yongxing;Zhao, Si;Zhang, Lu;Kang, Guiting;Wang, Feipeng;Yi, Hongmei;Ren, Wen;Li, Lu;He, Xiaoqing;Zhao, Jiuran;Yang, Jinxiao;Zhang, Chuanmao;Zhang, Bo;Krueger, Christopher J.;Krueger, Christopher J.;Krueger, Christopher J.;Xiao, Qianlin

作者机构：

期刊名称：NATURE PLANTS （ 影响因子：17.352； 五年影响因子：19.328 ）

ISSN： 2055-026X

年卷期： 2022 年 8 卷 1 期

页码：

收录情况： SCI

摘要： The ability to manipulate the genome in a programmable manner has illuminated biology and shown promise in plant breeding. Prime editing, a versatile gene-editing approach that directly writes new genetic information into a specified DNA site without requiring double-strand DNA breaks, suffers from low efficiency in plants(1-5). In this study, N-terminal reverse transcriptase-Cas9 nickase fusion performed better in rice than the commonly applied C-terminal fusion. In addition, introduction of multiple-nucleotide substitutions in the reverse transcriptase template stimulated prime editing with enhanced efficiency. By using these two methods synergistically, prime editing with an average editing frequency as high as 24.3% at 13 endogenous targets in rice transgenic plants, 6.2% at four targets in maize protoplasts and 12.5% in human cells was achieved, which is two- to threefold higher than the original editor, Prime Editor 3. Therefore, our optimized approach has potential to make more formerly non-editable target sites editable, and expands the scope and capabilities of prime editing in the future.

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