Investigating skeletal deformities in Murray cod (Maccullochella peelii): a comprehensive study combining transcriptomics and histology

文献类型： 外文期刊

第一作者： Song, Dan

作者： Song, Dan;Peng, Di;Cheng, Qiqun;Song, Dan;Zhu, Xinpeng

作者机构：

关键词： Murray cod; RNA-seq; Differentially expressed genes; Transcriptome; Deformation

期刊名称：AQUACULTURE INTERNATIONAL （ 影响因子：2.9； 五年影响因子：2.6 ）

ISSN： 0967-6120

年卷期： 2024 年

页码：

收录情况： SCI

摘要： The Murray cod (Maccullochella peelii), an economically and recologically valuable fish species, is extensively cultured in the aquaculture industry. However, the severe skeletal deformities that occur during the rearing process pose a significant challenge, profoundly impacting growth performance and economic sustainability. Despite recognition of these deformities, comprehensive studies investigating the underlying mechanisms of skeletal deformities in Murray cod remain scarce. To solve this problem, our study combines histological and molecular studies to understand the characteristics and mechanisms of these deformities. The results are as follows: (i) At the histological level, we observed notable abnormalities in the brain and muscle tissues of deformed Murray cod, including an enlarged ventricle and irregular muscle fiber structure. These observed abnormalities could potentially impact the skeletal development of Murray cod. (ii) At the molecular level, we performed a comprehensive transcriptome analysis of the brain and muscle tissues of normal and deformed Murray cod. Our analysis identified 8834 differentially expressed genes (DEGs), of which 3788 up-regulated genes and 4555 down-regulated genes. Enrichment analysis of GO and KEGG pathways showed that Cell Adhesion, Notch Signaling, Wnt Signaling, Cell Adhesion Molecules, The ECM-Receptor Interaction Pathway, and PI3K-Akt Signaling pathways may be involved in vertebral deformities. Notably, in deformed Murray cod, we observed significant changes in the expression of certain genes closely associated with skeletal development. For example, down-regulated expression of Fibronectin 1 (FN1) and Bone Morphogenetic Protein 2 (BMP2) was found in brain tissue. These genes may play a crucial role in the process of skeletal formation and development. At the same time, we verified through qRT-PCR to ensure the reliability of our data. In conclusion, our study provides a comprehensive understanding of Murray cod's skeletal deformities through histological and transcriptomic analyses and reveals the molecular mechanism of Murray cod's skeletal development. And provide insights to address these economically important anomalies.

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