The Endoplasmic Reticulum Cargo Receptor FgErv14 Regulates DON Production, Growth and Virulence in Fusarium graminearum

文献类型： 外文期刊

第一作者： Sun, Fengjiang

作者： Sun, Fengjiang;Zhang, Xuemeng;Wang, Chenyu;Zhang, Liyuan;Chen, Xiaochen;Liang, Yuancun;Chen, Lei;Zou, Shenshen;Dong, Hansong;Lv, Beibei;Zhang, Liyuan;Chen, Xiaochen;Chen, Lei;Zou, Shenshen;Dong, Hansong

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关键词： FgErv14; virulence; secretory pathway; ER cargo receptor; Fusarium graminearum

期刊名称：LIFE-BASEL （ 影响因子：3.251； ）

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年卷期： 2022 年 12 卷 6 期

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收录情况： SCI

摘要： Fusarium graminearum is a plant filamentous pathogenic fungi and the predominant causal agent of Fusarium head blight (FHB) in cereals worldwide. The regulators of the secretory pathway contribute significantly to fungal mycotoxin synthesis, development, and virulence. However, their roles in these processes in F. graminearum remain poorly understood. Here, we identified and functionally characterized the endoplasmic reticulum (ER) cargo receptor FgErv14 in F. graminearum. Firstly, it was observed that FgErv14 is mainly localized in the ER. Then, we constructed the FgErv14 deletion mutant (Delta Fgerv14) and found that the absence of the FgErv14 caused a serious reduction in vegetative growth, significant defects in asexual and sexual reproduction, and severely impaired virulence. Furthermore, we found that the Delta Fgerv14 mutant exhibited a reduced expression of TRI genes and defective toxisome generation, both of which are critical for deoxynivalenol (DON) biosynthesis. Importantly, we found the green fluorescent protein (GFP)-tagged FgRud3 was dispersed in the cytoplasm, whereas GFP-FgSnc1-PEM was partially trapped in the late Golgi in Delta Fgerv14 mutant. These results demonstrate that FgErv14 mediates anterograde ER-to-Golgi transport as well as late secretory Golgi-to-Plasma membrane transport and is necessary for DON biosynthesis, asexual and sexual reproduction, vegetative growth, and pathogenicity in F. graminearum.

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