The MADS-box transcription factor GmFULc promotes GmZTL4 gene transcription to modulate maturity in soybean

文献类型： 外文期刊

第一作者： Sun, Jingzhe

作者： Sun, Jingzhe;Fan, Yuhuan;Ma, Xiaofei;Ji, Yujia;Liu, Gaoyuan;Zhang, Xiaoming;Zhao, Lin;Sun, Jingzhe;Liu, Yucheng;Tian, Zhixi;Zheng, Yuhong;Wang, Shuming;Xue, Yongguo;Li, Yang

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关键词： flowering time; GmFULc; GmZTL4; maturity; photoperiod

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：9.3； 五年影响因子：9.3 ）

ISSN： 1672-9072

年卷期： 2024 年 66 卷 8 期

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收录情况： SCI

摘要： Flowering time and maturity are crucial agronomic traits that affect the regional adaptability of soybean plants. The development of soybean cultivars with early maturity adapted to longer days and colder climates of high latitudes is very important for ensuring normal ripening before frost begins. FUL belongs to the MADS-box transcription factor family and has several duplicated members in soybeans. In this study, we observed that overexpression of GmFULc in the Dongnong 50 cultivar promoted soybean maturity, while GmFULc knockout mutants exhibited late maturity. Chromatin immunoprecipitation sequencing (ChIP-seq) and RNA sequencing (RNA-seq) revealed that GmFULc could bind to the CArG, bHLH and homeobox motifs. Further investigation revealed that GmFULc could directly bind to the CArG motif in the promoters of the GmZTL3 and GmZTL4 genes. Overexpression of GmZTL4 promoted soybean maturity, whereas the ztl4 mutants exhibited delayed maturity. Moreover, we found that the cis element box 4 motif of the GmZTL4 promoter, a motif of light response elements, played an important role in controlling the growth period. Deletion of this motif shortened the growth period by increasing the expression levels of GmZTL4. Functional investigations revealed that short-day treatment promoted the binding of GmFULc to the promoter of GmZTL4 and inhibited the expression of E1 and E1Lb, ultimately resulting in the promotion of flowering and early maturation. Taken together, these findings suggest a novel photoperiod regulatory pathway in which GmFULc directly activates GmZTL4 to promote earlier maturity in soybean.

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