DNA Polymerase Theta Regulates the Growth and Development of Fusarium acuminatum and Its Virulence on Alfalfa

文献类型： 外文期刊

第一作者： Jing, Yuqing

作者： Jing, Yuqing;Yang, Jian;Lan, Bo;Li, Siyang;Guo, Qianqian;Yin, Kangquan;Ma, Renyi;Zhang, Qian;Du, Fang K.

作者机构：

关键词： Fusarium biology; DNA repair; Medicago sativa; root rot; mutant; pathogenicity; POLQ gene

期刊名称：AGRICULTURE-BASEL （ 影响因子：3.6； 五年影响因子：3.8 ）

ISSN：

年卷期： 2025 年 15 卷 11 期

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收录情况： SCI

摘要： Fusarium acuminatum is a major pathogenic fungus causing root rot in alfalfa (Medicago sativa). DNA polymerase theta is known to play a crucial role in repairing DNA double-strand breaks. However, its biological function in F. acuminatum remains unknown. In this study, the POLQ gene was deleted by homologous recombination using Agrobacterium tumefaciens-mediated transformation. Compared to the wild type (with the POLQ gene), the mutants (without the POLQ gene) showed significant phenotypic changes: they produced brown-yellow pigments instead of pink, slowed mycelial growth, and exhibited changes in macroconidia size and shape. The virulence of the mutants was greatly reduced, inducing only mild symptoms in alfalfa. In addition, FITC-WGA staining showed impaired spore germination and hyphal growth. These results suggest that POLQ is a key gene regulating growth and development of F. acuminatum, indicating that DNA repair may play an essential role in the pathogenicity of the pathogen in alfalfa. The POLQ gene could thus be a promising target for limiting F. acuminatum infections in alfalfa.

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