Transgenic Tobacco Expressing Zephyranthes Candida Agglutinin Showing Enhanced Resistance to Aphids

文献类型： 外文期刊

第一作者： Pang, Y

作者： Pang, Y;Shen, G;Qi, H;Tan, E;Sun, X;Tang, K

作者机构：

期刊名称：ENGINEERING IN LIFE SCIENCES （ 影响因子：2.678； 五年影响因子：2.704 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Aphid is one of the most serious, sap-sucking insect pests which cause significant losses of crop yields. The aim of this study is to investigate whether transgenic plants expressing Zephyranthes Candida agglutinin (ZCA) could confer enhanced resistance to aphids. Tobacco was transformed with a plasmid, pCAMBIAZCA, containing the marker genes nptll and gusA and the Zephyranthes Candida agglutinin gene (zca) via Agrobacterium tumefaciens-mediated transformation. Twenty-six independent transgenic plants were regenerated. Western blot analysis revealed ZCA expression at various levels in transgenic plants. Insect bioassay tests showed that transgenic plants expressing a high level of ZCA significantly inhibited the growth of the population of peach potato aphids (Myzus persicae Sulzer). This is the first report in which transgenic plants expressing ZCA conferred enhanced resistance to aphids. Our study suggests the zca gene could be a useful candidate for genetic engineering strategies in plants to provide aphid resistance.

分类号： Q

  • 相关文献
作者其他论文 更多>>

微信小程序