Construction of an insecticidal baculovirus expressing insect-specific neurotoxin AaIT

文献类型： 外文期刊

第一作者： Yao, B

作者： Yao, B;Pang, Y;Fan, YL;Zhao, RM;Yang, YC;Wang, TY

作者机构：

关键词： insect-specific neurotoxin AaIT;design and modification of gene;recombinant baculovirus

期刊名称：SCIENCE IN CHINA SERIES C-LIFE SCIENCES （ 影响因子：1.61； 五年影响因子：1.148 ）

ISSN： 1006-9305

年卷期： 1996 年 39 卷 2 期

页码：

收录情况： SCI

摘要： Considering the factors which affect gene transcription, translation and the stability of mRNA, without changing the amino acid composition of the encoded polypeptide, AaIT gene encoding insect-specific neurotoxin was designed and synthesized according to bias in codon choice, overall G+C content and G+C content of bases at the third position in codons of polyhedrin genes of baculovirus and of plant genes as well. AaIT gene was fused behind a synthetic gp67 signal sequence and then recombined into the genome of Trichoplusia ni nuclear polyhedrosis virus (TnNPV) by transfer Meter pSXIV VI(+)X3. The recombinant virus TnNPV-AaIT (occ(+)-gal(-)) was screened. The results of Southern blotting and SDS-PAGE demonstrated that AaIT gene had integrated into the genome of virus and expressed. Bioassays on the 3rd-instar Trichoplusia ni larvae showed that recombinant viruses TnNPV-AaIT could shorten the time of killing insect and improve the efficiency of killing agronomically important insects.

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