Improvement of Agrobacterium-mediated transformation efficiency and transgenic plant regeneration of Vitis vinifera L. by optimizing selection regimes and utilizing cryopreserved cell suspensions

文献类型： 外文期刊

第一作者： Li, P

作者： Li, P;Hanania, U;Sahar, N;Mawassi, M;Gafny, R;Sela, I;Tanne, E;Perl, A

作者机构：

关键词： cryopreservation;embryogenesis;grapevine;cell suspension;plant regeneration;transformation;Vitis vinifera;SOMATIC EMBRYOGENESIS;GENETIC-TRANSFORMATION;CULTURE;CULTIVARS;DENSITY;CALLUS;DNA

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Various concentrations of kanamycin and paromomycin were examined for their effect on embryogenic cell suspension viability, transformation efficiency, and transgenic plant regeneration. Paromomycin (10-25 mg/1) induced an earlier killing effect on cell suspensions than kanamycin (40-100 mg/l). Transformation efficiency and the number of embryos developed on selection medium were positively correlated with an increase in paromomycin concentrations from 10 to 20 or 25 mg/l. Paromomycin was more effective than kanamycin in selection of transformed cells and induction of embryo development during selection. Although no differences in transformation efficiency were observed between cryopreserved and non-cryopreserved cells, conversions of transformed cells to embryos and plantlets were significantly improved when cryopreserved cells were used as the target material. With the improved transformation procedure proposed, 65-80% of GUS-positive cells and embryos were produced with about 40% of transformed embryos regenerated into plants. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

分类号： Q94

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