Characterization of an intracellular cystatin homolog from the tick Haemaphysalis longicornis

文献类型： 外文期刊

第一作者： Zhou, Jinlin

作者： Zhou, Jinlin;Liao, Min;Ueda, Mami;Fujisaki, Kozo;Zhou, Jinlin;Gong, Haiyan;Xuan, Xuenan;Fujisaki, Kozo

作者机构：

关键词： Amino Acid Sequence;Animals;Cystatins chemistry metabolism;Gene Expression Regulation physiology;Molecular Sequence Data;Ticks genetics metabolism

期刊名称：VETERINARY PARASITOLOGY （ 影响因子：2.738； 五年影响因子：2.951 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Cystatins are tight-binding inhibitors of papain-like cysteine proteases. On the basis of amino acid sequencing, cystatins can be subdivided into three closely related families, family 1, family 2 and family 3. Among them, only family 1 cystatins are intracellular. In this report, a gene encoding family 1 cystatin from tick Haemaphysalis longicornis (Hlcyst-1) has been identified. Its full-length cDNA is 437 bp, including an intact ORF encoding an expected protein with 98 amino acids. Sequence analysis demonstrated that it has significant homology with the known family 1 cystatin. The recombinant protein was expressed in a GST-fused soluble form in Escherichia coli, and its inhibitory activity against papain, cathepsin L, and cathepsin B was identified by fluorogenic substrate analysis. This is the first report of an intracellular cystatin homolog from the tick H. longicornis.

分类号： S85

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