Recombinant Bivalent Vaccine against Foot-and-Mouth Disease Virus Serotype O/A Infection in Guinea Pig

文献类型: 外文期刊

第一作者: Yi, JZ

作者: Yi, JZ;Liu, MQ;Zhu, CZ;Zhang, Q;Sheng, ZT;Du, QY;Yan, WY;Zheng, ZX

作者机构:

关键词: foot-and-mouth disease;FMDV serotype O;FMDV serotype A;VP1 protein;bivalent vaccine;guinea pig

期刊名称:ACTA BIOCHIMICA ET BIOPHYSICA SINICA ( 影响因子:3.848; 五年影响因子:3.67 )

ISSN:

年卷期:

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收录情况: SCI

摘要: In this study,two DNA fragments encoding amino acid (141-160)-(21-40))-(141-160) of the VP1 of FMDV (foot-and-mouth disease virus) serotype O and (138-160)-(21-40)-(138-160) of the serotype A FMDV were chemically synthesized.These two tandem-repeat fragments were ligated and transfected into prokaryotic expression vector pTrcHis A to construct pTH-O-A.The other vector called pTH-O-scIgG-A was constructed similarly only that the two tandem-repeat DNA fragments were linked by the bovine-IgG heavy chain coding sequence.Guinea pigs immunized with the two bivalent vaccines pTH-O-A and pTH-O-scIgG-A showed both specific antibody activity and T cell proliferation responses.FMDV challenge tests showed that 85% and 70% of guinea pigs vaccinated twice with 200 mug of the fusion protein of pTH-O-A were protected from FMDV serotype O and serotype A infection respectively.70% and 57% of the guinea pigs immunized with the fusion protein of pTH-O-scIgG-A were protected from FMDV serotype O and serotype A infection respectively.

分类号: Q7

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