Cross-inhibition to heterologous foot-and-mouth disease virus infection induced by RNA interference targeting the conserved regions of viral genome

文献类型： 外文期刊

第一作者： Liu, MQ

作者： Liu, MQ;Chen, WZ;Ni, Z;Yan, WY;Fei, L;Jiao, Y;Zhang, J;Du, QY;Wei, X;Chen, JL;Liu, YM;Zheng, ZX

作者机构：

关键词： FMDV;RNA interference (RNAi);cross-inhibition;conserved region;viral genome;EMERGENCY VACCINATION;MAMMALIAN-CELLS;CONTACT TRANSMISSION;SECONDARY STRUCTURE;ANTIVIRAL IMMUNITY;REPLICATION;POLYMERASE;REDUCTION;SIRNA;IDENTIFICATION

期刊名称：VIROLOGY （ 影响因子：3.616； 五年影响因子：3.967 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： RNA interference (RNAi) is the process by which double-stranded RNA (dsRNA) directs sequence-specific degradation of messenger RNA in animal and plant cells. In mammalian cells, RNAi can be triggered by 21-23 nucleotide duplexes of small interfering RNA (siRNA). Strategies to inhibit RNA virus Multiplication based on the use of siRNAs have to consider the high genetic polymorphism exhibited by this group of virus. F ere we described a significant cross-inhibition of foot-and-mouth disease (FMD) virus (FMDV) replication in BHK-21 cells by siRNAs targeted to various conserved regions (5'NCR, VP4, VPg, POL, and YNCR) of the viral genome. The results showed that siRNAs generated in vitro by human recombinant dicer enzyme gave ail inhibition of 10- to 1000-fold in virus yield of both homologous (HKN/2002) and heterologom (CHA/99) isolates of FMDV serotype O at 48 h post-infection (hpi). The inhibition extended to at least 6 days postinfection. For serotype Asial, the virus yield in YNBS/58-infected cells examined at 12, 24, and 48 hpi decreased by ∼ 10-fold in cells pretreated with FKN/2002-specific siRNAs, but there was no significant decrease at 60 hpi. The inhibition was specific to FMDV replication, as no reduction was observed in virus yield of pseudorabies virus, an unrelated virus. Moreover, we also demonstrated an enhanced viral suppression could be achieved in BHK-21 cells with siRNA transfection after an infection had been established. These results suggested that siRNAs directed to several conserved regions of the FMDV genome Could inhibit FMDV replication in a cross-resistance manner, providing a strategy candicate to treat high genetic variability of FMDV. © 2005 Elsevier Inc. All rights reserved.

分类号： R373

  • 相关文献

[1]The VP1 S154D mutation of type Asia1 foot-and-mouth disease virus enhances viral replication and pathogenicity. Lian, Kaiqi,Yang, Fan,Zhu, Zixiang,Cao, Weijun,Jin, Ye,Liu, Huanan,Li, Dan,Zhang, Keshan,Guo, Jianhong,Liu, Xiangtao,Zheng, Haixue.

[2]Comparison of immune responses against foot-and-mouth disease virus induced by fusion proteins using the swine IgG heavy chain constant region or beta-galactosidase as a carrier of immunogenic epitopes. Li, GJ,Chen, WZ,Yan, WY,Zhao, K,Liu, MQ,Zhang, J,Fei, L,Xu, QX,Sheng, ZT,Lu, YG,Zheng, ZX.

[3]Tlr22 structure and expression characteristic of barbel chub, Squaliobarbus curriculus provides insights into antiviral immunity against infection with grass carp reovirus. Wang, Rong-hua,Li, Wei,Liu, Qiao-lin,Zeng, Ling-bing,Xiao, Tiao-yi,Fan, Yu-ding,Zeng, Ling-bing.

[4]The genomic diversity of Chinese porcine reproductive and respiratory syndrome virus isolates from 1996 to 2009. Xiao, Shaobo,Li, Bin,He, Kongwang.

[5]A one-step real-time reverse transcription-polymerase chain reaction detection of classical swine fever virus using a minor groove binding probe. Wen, Guoyuan,Yang, Jun,Luo, Qingping,Zhang, Rongrong,Wang, Hongling,Ai, Diyun,Luo, Ling,Shao, Huabin,Wen, Guoyuan,Luo, Qingping,Luo, Ling,Hu, Zhibin,Song, Nianhua.

[6]Validation of a loop-mediated isothermal amplification assay for visualised detection of wild-type classical swine fever virus. Zhang, Xing-Juan,Sun, Yuan,Qiu, Hua-Ji,Liu, Lihong,Belak, Sandor,Liu, Lihong,Belak, Sandor,Zhang, Xing-Juan.

[7]Effect of diacylglycerol on body weight: a meta-analysis. Xu, Tongcheng,Zhang, Zhiguo,Li, Duo,Xu, Tongcheng,Li, Xia,Ma, Xiaohang. 2008

[8]A Convenient Synthesis of D,L-Selenomethionine. Ran, X. G.,Cao, D. R.,Wang, L. Y.,Ran, X. G.,Lin, Y. C..

[9]The effect of different divalent cations on the reduction of hexavalent chromium by zerovalent iron. Hou, Meifang,Wan, Hongfu,Liu, Tinglin,Fan, Yanning,Liu, Xinming,Wang, Xugang.

[10]Reduction of iron oxides by Klebsiella pneumoniae L17: Kinetics and surface properties. Liu, Tong-xu,Li, Xiao-min,Li, Fang-bai,Zhang, Wei,Chen, Man-jia,Zhou, Shun-gui.

[11]Reduction, methylation, and translocation of arsenic in Panax notoginseng grown under field conditions in arsenic-contaminated soils. Ma, Jie,Mi, Yanhua,Li, Qiwan,Chen, Lu,Du, Lijuan,He, Lizhong,Ma, Jie,Lei, Mei.

[12]Manganese availability and microbial populations in the rhizosphere of wheat genotypes differing in tolerance to Mn deficiency. Marschner, P,Fu, QL,Rengel, Z.

[13]Adsorption recovery of Pd(II) from aqueous solutions by persimmon residual based bio-sorbent. Xie, Feng,Xie, Feng,Fan, Ruiyi,Yi, Qingping,Fan, Zaijun,Zhang, Qinglin,Luo, Zhengrong,Zhang, Qinglin,Luo, Zhengrong.

[14]Expression profiles of the p38 MAPK signaling pathway from Chinese shrimp I Fenneropenaeus chinensis in response to viral and bacterial infections. He, Yuying,Liu, Ping,Li, Jian,Wang, Qingyin,He, Yuying,Li, Jian,Yao, Wanlong. 2018

[15]RNA Interference of the Ecdysone Receptor Genes EcR and USP in Grain Aphid (Sitobion avenae F.) Affects Its Survival and Fecundity upon Feeding on Wheat Plants. Yan, Ting,Chen, Hongmei,Sun, Yongwei,Yu, Xiudao,Xia, Lanqin. 2016

[16]Effective inhibition of porcine transmissible gastroenteritis virus replication in ST cells by shRNAs targeting RNA-dependent RNA polymerase gene. Zhou, Jun-fang,Hua, Xiu-guo,Cui, Li,Zhu, Jian-guo,Miao, De-nian,Zou, Yong,He, Xi-zhong,Su, Wan-guo. 2007

[17]Arsenate reduction and methylation in the cells of Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 investigated with X-ray absorption near edge structure. Su, S. M.,Zeng, X. B.,Li, L. F.,Duan, R.,Bai, L. Y.,Li, A. G.,Wang, J.,Jiang, S.. 2012

[18]Identifying potential RNAi targets in grain aphid (Sitobion avenae F.) based on transcriptome profiling of its alimentary canal after feeding on wheat plants. Zhang, Min,Wang, Hui,Wang, Dahai,Ma, Youzhi,Xia, Lanqin,Zhou, Yuwen,Gao, Qiang,Jones, Huw Dylan. 2013

[19]Knockdown of Microplitis mediator Odorant Receptor Involved in the Sensitive Detection of Two Chemicals. Ren, Li-Yan,Zhang, Yong-Jun,Wu, Kong-Ming,Guo, Yu-Yuan,Li, Ke-Ming. 2012

[20]Double-stranded RNA in the biological control of grain aphid (Sitobion avenae F.). Wang, Dahai,Sun, Yongwei,Wang, Hui,Xia, Lanqin,Wang, Dahai,Liu, Qi,Li, Xia.

作者其他论文 更多>>

微信小程序