A PCR-based method to detect Sclerotium hydrophilum in infected rice leaf sheaths

文献类型： 外文期刊

第一作者： Huang, S. L.

作者： Huang, S. L.;Hu, C. J.;Li, Y. R.;Wei, Y. W.;Huang, S. L.;Huang, S. L.

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关键词： GenBank sequence number

期刊名称：AUSTRALASIAN PLANT PATHOLOGY （ 影响因子：1.599； 五年影响因子：1.708 ）

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收录情况： SCI

摘要： An oligonucleotide primer pair set based on internal transcribed spacer regions of rDNA for PCR, which gives the amplicon for only the DNA from Sclerotium hydrophilum was designed. Tests on the speci. city of the primers concerned showed that a single product of 540 bp was amplified from the DNA of S. hydrophilum but not from the DNA of any other species responsible for sheath disease in rice, suggesting that the primer pair could be used to develop a PCR-based diagnostic method to provide a rapid and accurate molecular detection of this pathogen in rice leaf sheath.

分类号： S4

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