Isolation and characterization of glyphosate-regulated genes in soybean seedlings

文献类型： 外文期刊

第一作者： Yu, Wancong

作者： Yu, Wancong;Zhang, Rui;Li, Runzhi;Guo, Sandui

作者机构：

关键词： transcriptome differential analysis;glyphosate-inducible genes;soybean;POLYMERASE-CHAIN-REACTION;RESISTANT CROPS;BACILLUS-THURINGIENSIS;DIFFERENTIAL-DISPLAY;MESSENGER-RNA;EXPRESSION;PLANT;IDENTIFICATION;PATHWAY;COTTON

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Glyphosate is a wide-used and broad-spectrum herbicide. The worldwide commercialization of glyphosale-resistant crop plants has made glyphosate much increased in use over the past decade. However, little information is available for the glyphosate-induced positive responses and the effects of this chemical on transcriptome in plants. In the present article, we investigate the transcriptional profile's changes induced by glyphosate in soybean seedlings. Twenty-four differentially expressed gene sequences were identified by mRNA differential display PCR (DDPCR). The web-based BLAST indicated that most of the glyphosate-induced genes are homologous to the known ESTs induced by abiotic factor stresses. The three differentially expressed genes, AG1, CT6 and GC10 were found to exhibit the time- and dose-dependent expression patterns. Transcription alternation was induced at a short time by a low dosage range of glyphosate treatment. The glyphosate-inducible effect on the gene expression was not genotype-depended in soybean plants. This study provides a preliminary basis for further study of global transcriptional profiles and the related physiological-biochemical responses to glyphosate stress in plants. The data is also beneficial to the development of a glyphosate-inducible system for the controlled expression of the target transgene in plants. (c) 2006 Published by Elsevier Ireland Ltd.

分类号： Q94

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