The pha2 gene cluster involved in Na+ resistance and adaption to alkaline pH in Sinorhizobium fredii RT19 encodes a monovalent cation/proton antiporter
文献类型: 外文期刊
第一作者: Yang, Lifu
作者: Yang, Lifu;Jiang, Juquan;Wei, Wei;Zhang, Bo;Wang, Lei;Yang, Susheng
作者机构:
关键词: Sinorhizobium fredii;pha2;monovalent cation;proton antiporter;BACILLUS-SUBTILIS;SYSTEM;MRP;HOMEOSTASIS;ADAPTATION;CHOLATE;EFFLUX;ROLES;C-125
期刊名称:FEMS MICROBIOLOGY LETTERS ( 影响因子:2.742; 五年影响因子:2.856 )
ISSN:
年卷期:
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收录情况: SCI
摘要: Sinorhizobium fredii RT19 can tolerate up to 0.6 M NaCl, whereas all its pha2-disrupted mutants, constructed by Tn5 mutagenesis, failed to grow in even the presence of 0.1 M NaCl. No growth difference was detected in pha2 mutants at a pH < 7.5 in the presence or absence of K+, but growth reduction was observed in the presence of K+ when pH > 7.5. The pha2 gene cluster was able to completely restore the growth of the pha2 mutants of S. fredii RT19 in 0.6 M NaCl. Measurement of monovalent cation intracellular content suggested that pha2 was involved in both Na+ (Li+) and K+ efflux. The pha2 mutants exhibited K+/H+, but no apparent Na+(Li+)/H+ antiporter activity in everted membrane vesicles. Taken together, these results indicated that the pha2 cluster of S. fredii RT19 encodes a monovalent cation/proton antiporter involved in resistance to Na+ and adaption to pH, which was very different from the pha1 cluster of Sinorhizobium meliloti, which encodes a K+/H+ antiporter.
分类号: Q93
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