The pha2 gene cluster involved in Na+ resistance and adaption to alkaline pH in Sinorhizobium fredii RT19 encodes a monovalent cation/proton antiporter

文献类型: 外文期刊

第一作者: Yang, Lifu

作者: Yang, Lifu;Jiang, Juquan;Wei, Wei;Zhang, Bo;Wang, Lei;Yang, Susheng

作者机构:

关键词: Sinorhizobium fredii;pha2;monovalent cation;proton antiporter;BACILLUS-SUBTILIS;SYSTEM;MRP;HOMEOSTASIS;ADAPTATION;CHOLATE;EFFLUX;ROLES;C-125

期刊名称:FEMS MICROBIOLOGY LETTERS ( 影响因子:2.742; 五年影响因子:2.856 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Sinorhizobium fredii RT19 can tolerate up to 0.6 M NaCl, whereas all its pha2-disrupted mutants, constructed by Tn5 mutagenesis, failed to grow in even the presence of 0.1 M NaCl. No growth difference was detected in pha2 mutants at a pH < 7.5 in the presence or absence of K+, but growth reduction was observed in the presence of K+ when pH > 7.5. The pha2 gene cluster was able to completely restore the growth of the pha2 mutants of S. fredii RT19 in 0.6 M NaCl. Measurement of monovalent cation intracellular content suggested that pha2 was involved in both Na+ (Li+) and K+ efflux. The pha2 mutants exhibited K+/H+, but no apparent Na+(Li+)/H+ antiporter activity in everted membrane vesicles. Taken together, these results indicated that the pha2 cluster of S. fredii RT19 encodes a monovalent cation/proton antiporter involved in resistance to Na+ and adaption to pH, which was very different from the pha1 cluster of Sinorhizobium meliloti, which encodes a K+/H+ antiporter.

分类号: Q93

  • 相关文献

[1]Co-overexpressing a Plasma Membrane and a Vacuolar Membrane Sodium/Proton Antiporter Significantly Improves Salt Tolerance in Transgenic Arabidopsis Plants. Pehlivan, Necla,Sun, Li,Jarrett, Philip,Mishra, Neelam,Zhang, Hong,Yang, Xiaojie,Chen, Lin,Shen, Guoxin,Kadioglu, Asim.

[2]Mutation of the rice narrow leaf1 gene, which encodes a novel protein, affects vein patterning and polar auxin transport. Qi, Jing,Bu, Qingyun,Li, Shuyu,Chen, Qian,Sun, Jiaqiang,Liang, Wenxing,Zhou, Yihua,Chu, Chengcai,Chen, Jinfeng,Chen, Mingsheng,Li, Chuanyou,Qian, Qian,Qi, Jing,Li, Shuyu,Chen, Qian,Liang, Wenxing,Li, Xugang,Ren, Fugang,Palme, Klaus,Li, Xugang,Ren, Fugang,Palme, Klaus,Zhao, Bingran.

[3]Effects of two efflux pump inhibitors on the drug susceptibility of Riemerella anatipestifer isolates from China. Li Ya-fei,Jiang Hong-xia,Zhang Ya-nan,Zhao Li-qing,Gu Peng,Wang Li-qiao,Zeng Zhen-ling,Xiang Rong,Sun Na. 2016

[4]A coherent transcriptional feed-forward motif model for mediating auxin-sensitive PIN3 expression during lateral root development. Chen, Qian,Liu, Yang,Maere, Steven,Van Isterdael, Gert,Xuan, Wei,Vassileva, Valya,Kitakura, Saeko,Wabnik, Krzysztof,Friml, Jiri,Beeckman, Tom,Vanneste, Steffen,Chen, Qian,Liu, Yang,Maere, Steven,Van Isterdael, Gert,Xuan, Wei,Vassileva, Valya,Kitakura, Saeko,Wabnik, Krzysztof,Friml, Jiri,Beeckman, Tom,Vanneste, Steffen,Chen, Qian,Li, Chuanyou,Liu, Yang,Lee, Eunkyoung,Lucas, Jessica,Sack, Fred,Xie, Zidian,Grotewold, Erich,Xie, Zidian,Grotewold, Erich,Vassileva, Valya,Kitakura, Saeko,Marhavy, Peter,Wabnik, Krzysztof,Benkova, Eva,Friml, Jiri,Marhavy, Peter,Geldner, Niko,Le, Jie,Fukaki, Hidehiro.

[5]Screening of high-yielding biocontrol bacterium Bs-916 mutant by ion implantation. Li, Dequan,Nie, Fengya,Wei, Lihui,Wei, Benqiang,Chen, Zhiyi.

[6]A Na+/H+ antiporter gene of the moderately halophilic bacterium Halobacillus dabanensis D-8(T): cloning and molecular characterization. Yang, LF,Jiang, JQ,Zhao, BS,Zhang, B,Feng, DQ,Lu, WD,Wang, L,Yang, SS.

[7]Fengycin antibiotics isolated from B-FS01 culture inhibit the growth of Fusarium moniliforme Sheldon ATCC 38932. Hu, Liang Bin,Shi, Zhi Qi,Zhang, Ting,Yang, Zhi Min.

[8]Effects of spaceflight on polysaccharides of Saccharomyces cerevisiae cell wall. Liu, Hong-Zhi,Wang, Qiang,Liu, Xiao-Yong,Tan, Sze-Sze.

[9]Carbon: Nitrogen: Phosphorus Stoichiometry in Fungi: A Meta-Analysis. Zhang, Ji,Zhang, Ji,Elser, James J.,Elser, James J.. 2017

[10]Tissue-Specific Regulation of the Contents and Correlations of Mineral Elements in Hens by Zinc Oxide Nanoparticles. Zhao, Yong,Zhang, Peng-Fei,Liu, Xin-Qi,Zhang, Wei-Dong,Huang, Ting-Ting,Zhao, Li,Hao, Zhi-Hui,Zhao, Yong,Feng, Yan-Ni,Li, Lan,Zhang, Peng-Fei,Zhang, Wei-Dong,Shen, Wei,Zhang, Hong-Fu,Zhang, Yu-Na.

[11]C:N:P stoichiometry in China's forests: From organs to ecosystems. Zhang, Jiahui,Liu, Congcong,Yang, Hao,Li, Meiling,Yu, Guirui,He, Nianpeng,Zhang, Jiahui,Yu, Guirui,He, Nianpeng,Zhao, Ning,Wilcox, Kevin,Yu, Qiang,He, Nianpeng,He, Nianpeng. 2018

[12]Proteomic analyses reveal differences in cold acclimation mechanisms in freezing-tolerant and freezing-sensitive cultivars of alfalfa. Chen, Jing,Han, Guiqing,Han, Guiqing,Shang, Chen,Li, Jikai,Zhang, Hailing,Liu, Fengqi,Wang, Jianli,Liu, Huiying,Zhang, Yuexue. 2015

[13]Construction of the Ontology-Based Agricultural Knowledge Management System. Zheng Ye-lu,He Qi-yun,Qian Ping,Li Ze. 2012

[14]The intelligent diagnostic system of vegetable diseases based on a fuzzy neural network. Wei, Qingfeng,Luo, Changshou,Cao, Chengzhong,Guo, Qiang. 2013

[15]RESEARCH ON THE METHOD OF GIS-BASED ANALYSIS ON AGRICULTURAL REGIONAL COMPETITIVE INDUSTRIES. Sun, Kaimeng,Zhu, Yeping,Xu, Yanchao. 2009

[16]Molecular cloning, expression, and enzymatic activity of a novel endogenous cellulase from the mulberry longicorn beetle, Apriona germari. Wei, Ya Dong,Lee, Kwang Sik,Gui, Zhong Zheng,Yoon, Hyung Joo,Kim, Iksoo,Zhang, Guo Zheng,Guo, Xijie,Sohn, Hung Dae,Jin, Byung Rae.

[17]Spread of recombinant Autographa californica nucleopolyhedrovirus in various tissues of silkworm Bombyx mori determined by real-time PCR. Zhang, Yi,Tian, Baozhong,Guo, Tingqing,Wang, Jianyang,Wei, Zhenguo,Lu, Changde,Zhang, Yi,Xia, Huanzhang,Tian, Baozhong,Wei, Zhenguo,Wang, Shengpeng.

[18]Eukaryotic expression and antimicrobial spectrum determination of the peptide tachyplesin II. Xu, Feng,Meng, Kun,Wang, Ya-Ru,Lu, Hui-Ying,Yang, Pei-Long,Yao, Bin,Xu, Feng,Wu, Ning-Feng,Fan, Yun-Liu.

[19]Realization of the Regional Advantageous Agricultural Industries Analysis System. Sun, Kaimeng. 2010

[20]Research on the Intelligent Control System of Greenhouse Based GPRS/GSM and PLC. Xu, Zhifu,Sun, Liping,Shi, Xiaoyan,Ye, Hongbao,Li, Dong. 2013

作者其他论文 更多>>