Cloning and Characterization of a GABA Receptor from Plutella xylostella (Lepidoptera: Plutellidae)

文献类型： 外文期刊

第一作者： Zhou, Xiao-Mao

作者： Zhou, Xiao-Mao;Wu, Qing-Jun;Zhou, Xiao-Mao;Zhang, You-Jun;Bai, Lian-Yang;Huang, Xiong-Ying

作者机构：

关键词： Plutella xylostella;GABA receptor;functional expression;Xenopus oocyte;abamectin;GATED CHLORIDE CHANNEL;DROSOPHILA-MELANOGASTER;CAENORHABDITIS-ELEGANS;SUBUNIT GENE;SITES;BRAIN;RDL

期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.381； 五年影响因子：2.568 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A full-length cDNA, with an open reading frame (ORF) of 1,449 bp, encoding a subunit of the gamma-aminobutyric acid (GABA)-activated chloride channel was isolated from Plutella xylostella (L.) (Lepidoptera: Plutellidae) (GenBank accession no. EF156251). The subunit gene encoded a 483-amino acid polypeptide that showed 84% sequence identity with DmRdl subunit (U02042) (Drosophila melanogaster resistant to dieldrin). When expressed in Xenopus laevis oocytes, the subunit assembled as a functional homomeric complex activated by GABA and abamectin in a dose-dependent manner. The EC50 value of GABA was 0.49 mM (0.41-0.58) (n = 5). However, the responses to abamectin were very robust, with an EC50 of 4.85 mu M (4.02-5.89) (n = 6), indicating that abamectin wits >100-fold more potent in activating chloride currents than GABA. The results suggest that this subunit is vital to the formation of a functional channel and contains the binding site of abamectin.

分类号： Q969.9

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