Molecular and biochemical characterization of a distinct tyrosinase involved in melanin production from Aeromonas media

文献类型: 外文期刊

第一作者: Wan, Xia

作者: Wan, Xia;Chai, Baozhong;Liao, Yi;Ye, Tao;Shen, Ping;Chen, Xiangdong;Wan, Xia;Su, Ying

作者机构:

关键词: Aeromonas media;Tyrosinase;Melanin;Diphenol oxidase;Monophenol oxidase;MARINOMONAS-MEDITERRANEA;STREPTOMYCES TYROSINASE;ESCHERICHIA-COLI;CLONING;GENE;IDENTIFICATION;ACTIVATION;EXPRESSION;SECRETION;MECHANISM

期刊名称:APPLIED MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:4.813; 五年影响因子:4.697 )

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年卷期:

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收录情况: SCI

摘要: A new tyrosinase was isolated from Aeromonas media strain WS and purified to homogeneity. The purified tyrosinase, termed TyrA, had a molecular mass of 58 kDa and an isoelectric point of 4.90. It exhibited optimal monophenol and diphenol oxidase activities under basic conditions (pH > 8.0). TyrA had a relatively higher affinity to diphenol substrate >l-dihydroxyphenylalanine (l-dopa) than many other tyrosinases. EDTA or glutathione notably inhibited the enzymatic activities of TyrA, whereas Triton X-100 and SDS activated them. The full-length TyrA gene was cloned, and it encodes a 518 amino acid protein with little similarities to other reported tyrosinases. However, the purified recombinant TyrA expressed in Escherichia coli demonstrated tyrosinase activity. These results suggest that TyrA is the first reported distinct tyrosinase involved in melanin production in the genus Aeromonas.

分类号: Q93

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