Optimization of fermentation medium and conditions for mycelial growth and water-soluble exo-polysaccharides production by Isaria farinosa B05

文献类型: 外文期刊

第一作者: Wang, P.

作者: Wang, P.;Jiang, X.;Jiang, Y.;Hu, X.;Jiang, Y.;Wang, P.;Hu, X.;Hwang, H.

作者机构:

关键词: exo-polysaccharides;Isaria farinosa;mycelium;optimization;submerged fermentation;SUBMERGED CULTURE-CONDITIONS;PAECILOMYCES-TENUIPES C240;CORDYCEPS-MILITARIS;BIOPOLYMER PRODUCTION;SCIENTIFIC REDISCOVERY;CHEMICAL-PROPERTIES;SINENSIS;AERATION;FUNGI;EXOPOLYSACCHARIDES

期刊名称:PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY ( 影响因子:2.162; 五年影响因子:1.901 )

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收录情况: SCI

摘要: The optimal fermentation medium and conditions for mycelial growth and water-soluble exo-polysaccharides production by Isaria farinosa B05 were investigated. The medium components and fermentation conditions were optimized according to the one at a time method, while the concentration of medium components was determined by the orthogonal matrix method. The results showed that the optimal fermentation medium was as follows: sucrose 3.5% (w/v), peptone 0.5%, yeast extract 0.2%, K2HPO4 0.1%, and MgSO4 0.05%. The suitable fermentation conditions were as follows: initial pH 7.0, temperature 25 degrees C, medium volume 75mL/250mL, inoculum volume 5% (v/v), time 5d. In such optimal nutrition and environmental conditions, the maximal mycelial yield was 2.124g/100mL after 4 day's fermentation, while maximal water-soluble exo-polysaccharides production reached 2.144g/L after 5 day's fermentation.

分类号: Q5

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