Development Of A Class-specific Monoclonal Antibody-based Elisa For Aflatoxins In Peanut

文献类型: 外文期刊

第一作者: Li, Peiwu

作者: Li, Peiwu;Zhang, Qi;Zhang, Wen;Zhang, Jinyang;Chen, Xiaomei;Jiang, Jun;Xie, Lihua;Zhang, Daohong

作者机构:

关键词: aflatoxins;class-specific;monoclonal antibody;enzyme-linked immunosorbent assay (elisa)

期刊名称:FOOD CHEMISTRY ( 影响因子:7.514; 五年影响因子:7.516 )

ISSN:

年卷期:

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收录情况: SCI

摘要: Three class-specific monoclonal antibodies against aflatoxins were screened by a designed strategy in which aflatoxin G_2 was used as competitor in the screening ELISA system. With a high cross-reactivity (65%) to aflatoxin G_2, antibody 10C9 had the most similar sensitivity for five aflatoxins (AFB], AFB_2, AFC], AFG_2 and AFM_1), whose I_(50) values were in a range of 2.1-3.2 ng ml~(-1). So, antibody 10C9 was selected to develop an ELISA for determination of aflatoxin B_1, B_2, G_1, G_2 and total of them in peanut samples. And spiked recoveries were from 87.5% to 102.0%. The results indicate that the ELISA developed can accurately determine total aflatoxins in samples of peanuts after the simple and rapid extraction procedure.

分类号: TS201`TS264`TS201.2

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