Recombination and expression of classical swine fever virus (CSFV) structural protein E2 gene in Chlamydomonas reinhardtii chroloplasts
文献类型: 外文期刊
第一作者: He, Dong-Mei
作者: He, Dong-Mei;Qian, Kai-Xian;Shen, Gui-Fang;Zhang, Zhi-Fang;Li, Yi-Nue;Su, Zhong-Liang;Shao, Hong-Bo
作者机构:
关键词: classical swine fever virus (CSFV);E2 gene;Chlanmydomonas reinhardtii;chloroplast transformatiom;anti-disease protein;biointerface;plant gene engineering;ENVELOPE GLYCOPROTEIN E2;HOG-CHOLERA;MARKER VACCINE;CHLOROPLAST;TRANSFORMATION;PROTECTION;RESPONSES;DISEASE;BIOLOGY;STRESS
期刊名称:COLLOIDS AND SURFACES B-BIOINTERFACES ( 影响因子:5.268; 五年影响因子:4.957 )
ISSN:
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收录情况: SCI
摘要: The expression of classical swine fever virus (CSFV) structural protein E2 in different vectors, which has been shown to carry critical epitopes, has been established. Here, we reported a Chlamydomonas reinhardtii chloroplast expression vector, P64E2, containing classical swine fever virus structural protein E2 gene, which was constructed and transferred to C. reinhardtii by biolistic bombardment method. The transformants were identified by PCR, Southern blotting, Western blotting after selecting on resistant media. ELISA quantification assay showed that the expressed E2 protein accumulated up to 1.5-2% of the total soluble protein. The results of the study on the immunological activity indicated that the protein E2 expressed in C. reinhardtii chloroplasts could elicit animal bodies to produce antibodies against protein E2. (C) 2006 Elsevier B.V. All rights reserved.
分类号: O6
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