Purification and characterization of an antioxidant protein from Ginkgo biloba seeds

文献类型： 外文期刊

第一作者： Huang, Wen

作者： Huang, Wen;Xie, Bijun;Tian, Binqiang;Deng, Qianchun;Huang, FengHong;Huang, Qingde;Shi, John;Xue, Sophia

作者机构：

关键词： ginkgo biloba seeds;protein;antioxidant activity;purification;physicochemical properties

期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：6.475； 五年影响因子：6.508 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： A new antioxidation protein named G4b was purified from Ginkgo biloba seed albumin. The results of chemical colorimetry and chemiluminescence methods showed that the hemi-inhibitory concentration (IC_(50)) of G4b on the hydroxyl radical were 78.1 μg/mL and 115.1 μg/mL, and the IC_(50) on DPPH was 100.7 μg/mL, while the IC_(50) on the superoxide anion and DNA injury were 23 μg/mL and 27 μg/mL, respectively, and the abundance of sulfur amino acids and aromatic amino acids contained in G4b might contribute to its strong antioxidation properties. C4b is a kind of homogeneous and novel protein with a molecular mass of 29,247 Da, consisting of two peptides with similar molecular weights linked by a disul-fide bond. Result of β-elimination reaction showed that G4b is connected with a small quantity of poly-saccharide by an oxygen-glucosidic bond.

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