Proteome analysis of maize seeds: the effect of artificial ageing

文献类型： 外文期刊

第一作者： Xin, Xia

作者： Xin, Xia;Lin, Xin-Hai;Chen, Xiao-Ling;Liu, Xu;Lu, Xin-Xiong;Lin, Xin-Hai;Zhou, Yuan-Chang

作者机构：

关键词： protease: 9001-92-7;EC 3.4.21.7;ATP: 111839-44-2;pyruvic acid: 127-17-3;glucose-6-phosphate dehydrogenase: 9001-40-5;EC 1.1.1.49;glucose 6-phosphate: 56-73-5;proteins: metabolism;upregulation;signaling;glycolysis;artificial aging;enzyme activity;tricarboxylic acid cycle;electron transport chain

期刊名称：PHYSIOLOGIA PLANTARUM （ 影响因子：4.5； 五年影响因子：4.576 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Previous understanding of the mechanism of seed ageing is largely based on observations on imbibed seeds rather than dry seeds. The present research was conducted to investigate whether seed ageing has effects on the dry seeds through proteome analysis. Maize (Zea mays cv. Dabaitou) seeds were artificially aged at 50 degrees C (13.58% moisture content) for 5 or 13 days, and the total protein was extracted from embryos of the dry seeds. Two-dimensional electrophoresis was performed and the differentially expressed proteins were identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. A total of 40 proteins were identified, in which 16 proteins were upregulated, indicating that artificial ageing affected the proteome of the dry seeds. Proteomic studies revealed that the signal transduction and transcription were disturbed by artificial ageing, which might lead to reduced protection against ageing. Artificial ageing also increased proteases and broke down stored proteins, impaired metabolism and energy supply, and ultimately resulted in seed deterioration. Proteins involved in metabolism and energy were the largest downregulated protein group, with regard to glycolysis, tricarboxylic acid cycle, the electron transport chain and oxidative phosphorylation. The downregulation of these proteins, together with reduction in the specific activity of glucose-6-phosphate dehydrogenase, and the content of glucose 6-phosphate, pyruvic acid and ATP in aged seeds, suggested the important roles of the mobilization of stored carbohydrates and energy supply in seed ageing and seed vigor. The present work provides new information about the proteomic changes during seed ageing and provides a possible mechanism for seed deterioration

分类号： Q945

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