Expression, purification and functional characterization of a recombinant 2,3-dihydroxybiphenyl-1,2-dioxygenase from Rhodococcus rhodochrous

文献类型： 外文期刊

第一作者： Xiong, Fei

作者： Xiong, Fei;Shuai, Jian-Jun;Peng, Ri-He;Tian, Yong-Sheng;Zhao, Wei;Yao, Quan-Hong;Xiong, Ai-Sheng;Xiong, Fei;Shuai, Jian-Jun

作者机构：

关键词： polychlorinated biphenyl: 92-52-4;pollutant;degradation;bioremediation

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A 2,3-dihydroxybiphenyl (2,3-DHBP) dioxygenase gene from a Rhodococcus sp. strain, named RrbphCI and involved in the degradation of polychlorinated biphenyls (PCBs), was synthesized. RrbphCI was expressed in Escherichia coli and its encoded enzyme was purified. SDS-PAGE analysis indicated that the size of the protein encoded by RrbphCI was about 32 kDa. The activity of the 2,3-DHBP dioxygenase was 82.8 U/mg when the substrate was 2,3-DHBP, with optimum pH 8.0 at 30A degrees C, and optimum temperature was 40A degrees C at pH 8.0. The RrbphCI gene was transformed into Pseudomonas putida strain EG11, to determine the ability of the enzyme to degrade 2,3-DHBP. The wild type EG11 degraded 61.86% of supplied 2,3-DHBP and the transformed EG11 (hosting the RrbphCI gene) utilized 52.68% after 2 min of treatment at 30A degrees C. The overexpressed and purified enzyme was able to degrade 2,3-DHBP. The 2,3-DHBP dioxygenase is a key enzyme in the PCB degradation pathway. RrbphCI and its encoded 2,3-DHBP dioxygenase may have transgenic applications in bioremediation of PCBs.

分类号： Q7

  • 相关文献

[1]Genome Sequence of Acinetobacter calcoaceticus PHEA-2, Isolated from Industry Wastewater. Zhan, Yuhua,Yan, Yongliang,Zhang, Wei,Yu, Haiying,Chen, Ming,Lu, Wei,Ping, Shuzhen,Peng, Zixin,Yuan, Menglong,Zhou, Zhengfu,Lin, Min,Elmerich, Claudine.

[2]Fluoride toxicity to Chinese sturgeon (Acipenser sinensis) larvae and fluoride concentrations in the Yangtze Estuary. Zhuang, P.,Shi, X.,Zhang, L.,Feng, G.,Liu, J.,Zhuang, P.,Shi, X.,Wang, R.,Shi, X..

[3]Biodegradation of pentachloronitrobenzene by Arthrobacter nicotianae DH19. Wang, Y.,Gao, J.,Wang, C.,Li, A..

[4]Mineralization of chlorpyrifos by co-culture of Serratia and Trichosporon spp.. Xu, Gangming,Li, Yingying,Zheng, Wei,Peng, Xiang,Li, Wen,Yan, Yanchun.

[5]Fast atrazine degradation by the mixed cultures enriched from activated sludge and analysis of their microbial community succession. Zhou, Qingxin,Chen, Leilei,Wang, Junhua,Qiu, Jiying,Liu, Xiaoyong,Zhang, Xiang,Chen, Xiangyan,Wang, Zhibin,Ni, Shouqing,Wang, Zhibin.

[6]Bioremediation potential of glyphosate-degrading Pseudomonas spp. strains isolated from contaminated soil. Zhu, Jianyi,Liu, Shengnan,Gao, Han,Zhou, Xiaogang,Tao, Ke.

[7]Influence of environmental factors on degradation of carbendazim by Bacillus pumilus strain NY97-1. Zhang, Lizhen,Qiao, Xiongwu,Ma, Liping,Zhang, Lizhen.

[8]Isolation, Colonization, and Chlorpyrifos Degradation Mediation of the Endophytic Bacterium Sphingomonas Strain HJY in Chinese Chives (Allium tuberosum). Feng, Fayun,Ge, Jing,Cheng, Jinjin,Yu, Xiangyang,Feng, Fayun,Ge, Jing,Zhong, Jianfeng,Yu, Xiangyang,Li, Yisong.

[9]Transport via xylem of atrazine, 2,4-dinitrotoluene, and 1,2,3-trichlorobenzene in tomato and wheat seedlings. Su, Yu H.,Liang, Yong C.,Liang, Yong C..

[10]Removal of nutrients and veterinary antibiotics from swine wastewater by a constructed macrophyte floating bed system. Xian, Qiming,Chang, Zhizhou,Xian, Qiming,Hu, Lixia,Chen, Hancheng,Zou, Huixian.

[11]Identification of quantitative trait loci for Cd and Zn concentrations of brown rice grown in Cd-polluted soils. Zhang, Xiaoqin,Wang, Huizhong,Qian, Qian,Xue, Dawei,Zhang, Xiaoqin,Zhang, Guoping,Guo, Longbiao,Zeng, Dali,Dong, Guojun,Qian, Qian.

[12]Protective function of narrow grass hedges on soil and water loss on sloping croplands in Northern China. Xiao, Bo,Wang, Qing-hai,Wu, Ju-ying,Huang, Chuan-wei,Yu, Ding-fang,Xiao, Bo.

[13]Threshold of Soil Olsen-P in Greenhouses for Tomatoes and Cucumbers. Wu, Xue-Ping,Wu, Hui-Jun,Wang, Xiao-Bin,Li, Yin-Kun,Zhang, Yan-Cai,Li, Ruo-Nan,Wang, Li-Ying,Zhai, Cai-Xia,Chen, Li-Li,Wu, Xue-Ping,Wu, Hui-Jun,Wang, Xiao-Bin.

[14]Assessment of pollutant mean concentrations in the Yangtze estuary based on MSN theory. Ren, Jing,Gao, Bing-Bo,Ren, Jing,Gao, Bing-Bo,Wang, Jin-Feng,Fan, Hai-Mei,Zhang, Yao,Zhang, Zhi-Hong,Wang, Jin-Feng.

[15]Wheat Growth and Photosynthesis as Affected by Oxytetracycline as a Soil Contaminant. Li Zhao-Jun,Xie Xiao-Yu,Zhang Shu-Qing,Liang Yong-Chao.

[16]Comparative Study on Disturbed and Undisturbed Soil Sample Incubation for Estimating Soil Nitrogen-Supplying Capacity. Li, Shi-Qing,Xu, Bing-Cheng,Zhao, Kun,Li, Shi-Qing,Xu, Bing-Cheng,Zhao, Kun,Li, Shi-Qing,Li, Sheng-Xiu,Zhao, Kun,Lu, Hong-Ling.

[17]Isolation and identification of a phosphate-solubilizing bacterium Pantoea stewartii subsp. stewartii g6, and effects of temperature, salinity, and pH on its growth under indoor culture conditions. Hu, Xiao-Juan,Zhang, Jun,Yang, Yu-Feng,Hu, Xiao-Juan,Li, Zhuo-Jia,Cao, Yu-Cheng,Gong, Ying-Xue.

[18]A new, simple and rapid HPLC method for determination of chlortetracycline in pig solid manure. Wang, Ran,Wang, Ran,Wang, Tian.

[19]INFLUENCE OF THE Nocardia sp. C-14-1 REMEDIATION ON THE GROWTH OF POTTED PLANTS IN CONTAMINATED SOIL AND ON MICROORGANISM BIO-DIVERSITY. Ma, Hai-Juan,Ma, Hai-Juan,Li, Guang-Ming,Zhang, Ya-Lei,Fang, Ping. 2011

[20]Bioremediation of Eutrophic Water by the Controlled Cultivation of Water Hyacinths. Zhang, Yingying,Zhang, Zhiyong,Wang, Zhi,Liu, Haiqin,Wen, Xuezheng,Qin, Hongjie,Yan, Shaohua,Zhang, Yingying,Rittmann, Bruce E.. 2016

作者其他论文 更多>>

微信小程序