Analysis of Genetic Diversity and Phylogenetic Relationship of Kenaf Germplasm by SRAP

文献类型： 外文期刊

第一作者： Qi, Jianmin

作者： Qi, Jianmin;Li, Aiqing;Li, Aiqing;Su, Jianguang;Liu, Aimin

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关键词： kenaf;srap;genetic diversity;dna molecular marker

期刊名称：JOURNAL OF NATURAL FIBERS （ 影响因子：5.323； 五年影响因子：4.183 ）

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收录情况： SCI

摘要： The genetic diversity and phylogenetic relationship were analyzed by sequence-related amplified polymorphism (SRAP) marker system on 84 varieties of kenaf fHibiscus cannabinus L.) germplasm collected from 26 countries and regions around the world. Twenty-six of total 225 SRAP primer combinations showed polymorphisms. Analysis of 84 kenaf varieties by using the 26polymorphic primer combinations had identified a total of 329 reproducible bands. Among them, 248 bands (75.4%) were polymorphic. The number of polymorphic fragments produced by an individual primer combination ranged from 5 to 15, with an average of 9.54 bands. DNA fragment size ranged from 300 bp to 1500 bp. Cluster analysis of the SRAP fingerprints clearly separated the 84 varieties into three distinct groups: cultivar, wild, and intermediate types. The cultivars were further delineated into four subgroups. High genetic similarity was found among varieties from the same geographic region, resulting from the fact that kenaf is a plant dominated by cross-pollination. This research indicated that SRAP molecular marker could be used to perform kenaf varieties identification, genetic diversity and phylogenetic relationship assessment, and germplasm evaluation.

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