Rapid identification of deer products by multiplex PCR assay

文献类型： 外文期刊

第一作者： Zha, Dai-Ming

作者： Zha, Dai-Ming;Xing, Xiu-Mei;Yang, Fu-He;Zha, Dai-Ming;Zha, Dai-Ming;Xing, Xiu-Mei;Yang, Fu-He;Zha, Dai-Ming;Xing, Xiu-Mei;Yang, Fu-He

作者机构：

关键词： multiplex PCR;deer products;species identification;D-loop region;16SrDNA

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Attempts were made to establish one-step multiplex PCR assay for the identification of the widely used species in deer products (sika deer, wapiti, red deer and reindeer). Primers were designed from tandem repeat region of D-loop and well-conserved region of 16S rDNA after alignment of the available sequences in the GenBank database. The primers generated specific fragments of 307 bp in length for sika deer, 307 and 246 bp for wapiti, 272 bp for Tarim red deer, 230 bp for red deer and 141 bp for reindeer, respectively. The detection limit was 0.05 ng for sika deer and wapiti, 0.1 ng for Tarim red deer, 0.5 ng for red deer and 0.02 ng for reindeer. The results demonstrated that the fraudulent phenomenon is epidemic in the substitution of deer products, in especially antler, penis, foetus and tendon products. Hence, this multiplex PCR provided a useful and sensitive technique to identify the sources of deer products.

分类号： TS2

  • 相关文献

[1]A multiplex PCR assay for fraud identification of deer products. Zha, Daiming,Xing, Xiumei,Yang, Fuhe,Zha, Daiming,Zha, Daiming,Xing, Xiumei,Yang, Fuhe,Zha, Daiming,Xing, Xiumei,Yang, Fuhe. 2010

[2]ISOLATION AND CHARACTERIZATION OF CELLULOSE-DEGRADING BACTERIA FROM PRIMEVAL FOREST SOIL. Liu, Xiaolu,Yang, Liuqing,Liu, Yongzhi,Shanqi, Mike,Lv, Le,Xu, Qianqian,Yan, Hai,Zhang, Chunqiu.

[3]Efficient Identification of the Forest Tree Species in Aceraceae Using DNA Barcodes. Yu-Wei Han,Dong Duan,Xiong-Feng Ma,Yun Jia,Zhan-Lin Liu,Gui-Fang Zhao,Zhong-Hu Li. 2016

[4]The complete mitochondrial genome sequence of Hemibagrus sp. (Siluriformes: Bagridae), molecular data for species identification. Zhou, Can-Lin,Zhou, Can-Lin,Tian, Juan,Yang, Chang-Geng. 2016

[5]Scylla paramamosain (Estampador) the most common mud crab (Genus Scylla) in China: evidence from mtDNA. Ma, Ling-Bo,Zhang, Feng-Ying,Ma, Chun-Yan,Qiao, Zhen-Guo. 2006

[6]Complete mitochondrial genome of the sea cucumber Stichopus sp and its application in the identification of this species. Fan, Sigang,Hu, Chaoqun,Zhang, Lvping,Sun, Hongyan,Luo, Peng,Fan, Sigang,Wen, Jing. 2012

[7]Genetics and phylogeny of genus Coilia in China based on AFLP markers. Yang Qiaoli,Han Zhiqiang,Gao Tianxiang,Sun Dianrong,Xie Songguang,Lin Longshan. 2010

[8]Identification and evaluation of Aspergillus tubingensis as a potential biocontrol agent against grey mould on tomato. Zhao, Juan,Liu, Weicheng,Liu, Dewen,Lu, Caige,Zhang, Dianpeng,Wu, Huiling,Dong, Dan,Meng, Lingling. 2018

[9]Existence of species complex largely reduced barcoding success for invasive species of Tephritidae: a case study in Bactrocera spp.. Jiang, F.,Li, Z. H.,Jin, Q.,Zhang, A. B.,Liang, L..

[10]The complete mitochondrial genome sequence of Takifugu flavidus (Tetraodontiformes: Tetrodontidae). Liu, Yongfu,Li, Chao,Tong, Aiping,Zhou, Qin,Liu, Haijin.

[11]Identification of Forensically Important Blow Flies (Diptera: Calliphoridae) in China Based on COI. Meng, Fanming,Ren, Lipin,Wang, Ziyue,Guo, Yadong,Finkelbergs, Dmitrijs,Cai, Jifeng,Deng, Jianqiang,Chen, Chao.

[12]DNA barcoding reveals mislabeling of imported fish products in Nansha new port of Guangzhou, Guangdong province, China. Yan, Shuai,Lai, Guiyan,Li, Li,Xiao, Hao,Zhao, Ming,Wang, Ming,Wang, Ming.

[13]Duplex PCR approach for the detection and quantification of donkey, horse and mule in raw and heat-processed meat products. Chen, Ailiang,Chen, Gang,Zhao, Yan,Yang, Shuming,Chen, Ailiang,Chen, Gang,Zhao, Yan,Yang, Shuming,Wei, Chengbin.

[14]Species identification of fish maw (Porcupinefish) products sold on the market using DNA sequencing of 16S rRNA and COI genes. Zeng, Ling,Chen, Ziming,Wen, Jing,Sun, Yulin,Chen, Daohai,Zhao, Juan,Fan, Sigang,Xu, Youhou. 2018

[15]The complete mitochondrial genome sequence of Pampus argenteus ( Perciformes: Stromateidae). Sun, Dandan,Cheng, Qiqun,Qiao, Huiying,Chen, Ying,Sun, Dandan,Qiao, Huiying,Chen, Ying. 2016

[16]Species identification of Chinese sturgeon using acoustic descriptors and ascertaining their spatial distribution in the spawning ground of Gezhouba Dam. Tao JiangPing,Qiao Ye,Chang JianBo,Tao JiangPing,Qiao Ye,Chang JianBo,Tao JiangPing,Tan XiChang,Tao JiangPing,Tan XiChang. 2009

[17]Isolation and Identification of a Pathogen of Silkworm Bombyx mori. Tao, Heng-Ping,Shen, Zhong-Yuan,Zhu, Feng,Xu, Xiao-Fang,Tang, Xu-Dong,Shen, Zhong-Yuan,Xu, Li.

[18]PhoR sequences as a phylogenetic marker to differentiate the species in the Bacillus subtilis group. Guo, Qinggang,Li, Shezeng,Lu, Xiuyun,Li, Baoqing,Dong, Weixin,Ma, Ping,Stummer, Belinda.

[19]A species-specific primer pair for distinguishing between Paramisgurnus dabryanus and Misgurnus anguillicaudatus based on mitochondrial DNA polymorphisms. Liu, Yongfu,Liu, Yongfu,Liu, Haijin,Hou, Jilun,Wang, Guixing,Zhang, Xiaoyan.

[20]Complete mitochodrial genome of Vanmanenia pingchowensis (Cypriniformes, Cyprinidae). Liang, Zhiqiang,Wei, Qiwei,Liang, Zhiqiang,Wei, Qiwei,Liang, Zhiqiang,Wang, Chongrui,Wu, Yuanan,Li, Hong,Yuan, Xiping.

作者其他论文 更多>>

微信小程序