Rapid identification of deer products by multiplex PCR assay

文献类型： 外文期刊

第一作者： Zha, Dai-Ming

作者： Zha, Dai-Ming;Xing, Xiu-Mei;Yang, Fu-He;Zha, Dai-Ming;Zha, Dai-Ming;Xing, Xiu-Mei;Yang, Fu-He;Zha, Dai-Ming;Xing, Xiu-Mei;Yang, Fu-He

作者机构：

关键词： multiplex PCR;deer products;species identification;D-loop region;16SrDNA

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Attempts were made to establish one-step multiplex PCR assay for the identification of the widely used species in deer products (sika deer, wapiti, red deer and reindeer). Primers were designed from tandem repeat region of D-loop and well-conserved region of 16S rDNA after alignment of the available sequences in the GenBank database. The primers generated specific fragments of 307 bp in length for sika deer, 307 and 246 bp for wapiti, 272 bp for Tarim red deer, 230 bp for red deer and 141 bp for reindeer, respectively. The detection limit was 0.05 ng for sika deer and wapiti, 0.1 ng for Tarim red deer, 0.5 ng for red deer and 0.02 ng for reindeer. The results demonstrated that the fraudulent phenomenon is epidemic in the substitution of deer products, in especially antler, penis, foetus and tendon products. Hence, this multiplex PCR provided a useful and sensitive technique to identify the sources of deer products.

分类号： TS2

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