Evaluation of High-Resolution Melting for Gene Mapping in Rice

文献类型: 外文期刊

第一作者: Li, Jinshan

作者: Li, Jinshan;Wang, Xuming;Dong, Ruixian;Yang, Yong;Zhou, Jie;Yu, Chulang;Cheng, Ye;Yan, Chengqi;Chen, Jianping;Li, Jinshan;Dong, Ruixian

作者机构:

关键词: gene;simple sequence repeat marker: SSR marker;sequence-tagged site marker: STS marker;genotype;polymorphic melting curve

期刊名称:PLANT MOLECULAR BIOLOGY REPORTER ( 影响因子:1.595; 五年影响因子:2.042 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: In this study, high-resolution melting (HRM) analysis was evaluated for gene mapping in rice with sequence-tagged site (STS) and simple sequence repeat (SSR) markers. A total of 103 out of 353 normal STS and SSR markers revealed polymorphic melting curves among the parental genotypes, and 12 of these were successfully used to genotype the F-2 mapping population for HRM analysis. Additional electrophoresis findings demonstrated that HRM genotyping matched with traditional electrophoresis results. To optimize the HRM-marker screening efficiency, different HRM reaction conditions were evaluated. A 5-mu l touchdown-polymerase chain reaction (PCR) system provided no significant improvement in screening efficiency but in a 10-mu l touchdown-PCR system, the marker screening efficiency increased by 75%. Twenty-one markers were obtained for mapping purposes under the optimized reaction conditions. This study indicates that HRM analysis can speed up the gene mapping progress in rice, while saving a lot of manpower.

分类号: Q94

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