Single nucleotide polymorphism of CACNA2D1 gene and its association with milk somatic cell score in cattle

文献类型: 外文期刊

第一作者: Yuan, Zheng Rong

作者: Yuan, Zheng Rong;Li, Jiao;Liu, Li;Zhang, Lu Pei;Zhang, Li Min;Chen, Cui;Chen, Xiao Jie;Gao, Xue;Li, Jun Ya;Chen, Jin Bao;Gao, Hui Jiang;Xu, Shang Zhong

作者机构:

关键词: aspartic acid: 617-45-8;glycine: 56-40-6;genotype;allelic variation;disease resistance;backfat thickness

期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )

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收录情况: SCI

摘要: The objective of the present study was to identify polymorphisms of the CACNA2D1 gene, and to analyze associations between these polymorphisms and mastitis in several cattle breeds. Through PCR-RFLP methods and DNA sequencing, an allelic variant corresponding to the A -> G mutations and Aspartic (Asp) to Glycine (Gly) amino acid replacement at positions 526745 in the exon 25 of bovine CACNA2D1 gene could be detected. Two alleles, A and G, and three genotypes, AA, AG and GG were defined. Genetic character in the studied populations indicated that the A526745G loci of CACNA2D1 gene was moderate polymorphism and fitted with Hardy-Weinberg equilibrium (P > 0.05). The effects of CACNA2D1 polymorphisms on somatic cell score (SCS) were analyzed and significant association was found between A526745G and SCS. The mean of genotype GG was significantly lower than those of genotype AG and AA (P = 0.0469). Information provided in this research could be useful in further studies to determine the role of CACNA2D1 gene in the mastitis resistance.

分类号: Q7

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