Selective sample cleanup by immunoaffinity chromatography for determination of fenvalerate in vegetables

文献类型： 外文期刊

第一作者： Wang, Yanru

作者： Wang, Yanru;Zhang, Qi;Li, Peiwu;Zhang, Wen;Li, Ying;Ding, Xiaoxia;Wang, Yanru;Li, Peiwu;Ding, Xiaoxia;Zhang, Qi;Wang, Yanru;Li, Peiwu;Zhang, Wen;Li, Ying;Ding, Xiaoxia

作者机构：

关键词： Fenvalerate;Gas chromatography (GC);Immunoaffinity chromatography;Vegetables

期刊名称：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES （ 影响因子：3.205； 五年影响因子：3.068 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： This paper describes the establishment of an immunoaffinity chromatography (IAC) for selective extraction of fenvalerate from vegetable samples. The IAC column was constructed by covalently coupling monoclonal antibody (mAb) against fenvalerate to CNBr-activated Sepharose 4B and packed into a cartridge. The extraction conditions were carefully optimized, including loading, washing and eluting solutions. Under the optimal conditions, the IAC column was able to capture fenvalerate with the maximum capacity of 4000ng. An average recovery of 94.5% and a RSD of 8.8% were obtained with six IAC columns prepared on six different days. Three vegetable samples spiked with fenvalerate at four different concentrations were extracted with IAC column and determined by gas chromatography with electron capture detection (GC-ECD). Chromatograms of final extracts were clean and fenvalerate could be easily detected without the interferences. The extraction recoveries and RSD were 74.7-96.5% and 2.5-5.2%, respectively, and the calculated limit of detection of the whole method was 0.008-0.012ngg~(-1).

分类号： Q503

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