Derivation and Characterization of Ovine Embryonic Stem-Like Cell Lines in Semi-defined Medium Without Feeder Cells

文献类型： 外文期刊

第一作者： Zhao, Yuncheng

作者： Zhao, Yuncheng;Lin, Jiapeng;Wang, Liqin;Chen, Bo;Zhou, Chuan;Chen, Tong;Guo, Meiying;He, Sangang;Zhang, Ning;Liu, Chenxi;Liu, Mingjun;Huang, Juncheng;Zhao, Yuncheng;Lin, Jiapeng;Wang, Liqin;Chen, Bo;Zhou, Chuan;Chen, Tong;Guo, Meiying;He, Sangang;Zhang, Ning;Liu, Chenxi;Liu, Mingjun;Huang, Juncheng

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期刊名称：JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL GENETICS AND PHYSIOLOGY （ 影响因子：1.28； 五年影响因子：1.302 ）

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收录情况： SCI

摘要： Domestic animal embryonic stem (ES) cells would provide an invaluable research tool for genetic breeding and the production of transgenic animals. Unfortunately, authentic domestic animals ES cells have not been established despite progress made over more than two decades. Here, we show that ovine ES-like cells can be efficiently derived and propagated in a semi-defined medium that contains N2, B27, GSK3 inhibitor (CHIR99021), and basic fibroblast growth factor (bFGF). These ovine ES-like cells had a characteristic three-dimensional appearance, showed a bFGF dose-dependence, expressed specific markers such as alkaline phosphatase (AP), Oct-4, Sox2, Nanog and can be maintained for 30 passages. Moreover, these cells differentiated in vitro into neuronal cells, and formed teratomas containing a variety of different tissues including cartilage and neural tissue when injected into kidney capsules of severe combined immunodeficiency (SCID) mice. But the cell lines fail to contribute to embryonic development upon blastocyst transplantation. To our knowledge, this is the first experiment to use semi-defined medium without feeder-cells to derive ES-like cells from ovine blastocysts, and opens the door to deriving authentic ES cells from domesticated ungulates. J. Exp. Zool. 315:639-648, 2011

分类号： Q

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