Proteomics Analysis of Porcine Serum Proteins by LC-MS/MS after Foot-and-Mouth Disease Virus (FMDV) Infection

文献类型： 外文期刊

第一作者： Liu, Yongjie

作者： Liu, Yongjie;Zhang, Keshan;Zheng, Haixue;Shang, Youjun;Guo, Jianhong;Tian, Hong;Lu, Guodong;Jin, Ye;He, Jijun;Cai, Xuepeng;Li, Xiangtao

作者机构：

关键词： foot and mouth disease virus infection;liquid chromatography-tandem mass spectrometry;porcine serum;proteomics analysis

期刊名称：JOURNAL OF VETERINARY MEDICAL SCIENCE （ 影响因子：1.267； 五年影响因子：1.348 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： To analyze serum proteomics differences between normal and foot and mouth disease virus (FMDV)-infected piglets, an analytical method based on liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used. Samples of venous blood were collected before and after FMDV infection and high abundance serum albumin was removed using a commercial kit. After trypsin digestion, serum samples were processed with LC-MS/MS. Proteins were identified by peptide mass fingerprinting. We found that apolipoprotein A-IV precursor, haptoglobin and probable chemoreceptor glutamine deamidase cheD appeared after FMDV infection in the same piglet. This is believed to be the first time that serum proteomics analysis by LC-MS/MS after FMDV infection has been performed, and our results may provide further information about biomarkers for early diagnosis of FMD in piglets.

分类号： S85

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