Recombinant microsatellite amplification: a rapid method for developing simple sequence repeat markers

文献类型： 外文期刊

第一作者： Wu, Bin

作者： Wu, Bin;Lu, Ping;Zhang, Zongwen;Zhang, Zongwen

作者机构：

关键词： Microsatellites;Locus-specific simple sequence repeat;Cultivated oats;Anchored primers

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Recombinant microsatellite (simple sequence repeat; SSR) amplification is a technique by which DNA sequences flanking microsatellites can be isolated rapidly on a large scale. The approach selectively amplifies microsatellite-containing sequences and recombines the amplicons by redigestion and ligation, in order to increase the yield of microsatellite flanks per clone two-fold and to further increase selectivity of amplification. Since this method does not require prior knowledge of the genomic sequence, it is especially useful for species for which abundant genomic sequences are not available. The feasibility of this approach was demonstrated by developing SSR markers in cultivated oats.

分类号： Q94

  • 相关文献

[1]Genome-Wide Discovery of Microsatellite Markers from Diploid Progenitor Species, Arachis duranensis and A. ipaensis, and Their Application in Cultivated Peanut (A. hypogaea). Zhao, Chuanzhi,Qiu, Jingjing,Wang, Jiangshan,Ren, Xuezhen,Xia, Han,Wan, Shubo,Wang, Xingjun,Zhao, Chuanzhi,Qiu, Jingjing,Wang, Jiangshan,Ren, Xuezhen,Xia, Han,Wan, Shubo,Wang, Xingjun,Qiu, Jingjing,Ma, Changle,Wang, Xingjun,Agarwal, Gaurav,Guo, Baozhu,Agarwal, Gaurav,Varshney, Rajeev K.,Pandey, Manish K.,Bertioli, David J.. 2017

[2]Quantitative trait loci mapping of adult-plant resistance to powdery mildew in Chinese wheat cultivar Lumai 21. Caixia Lan,Xiaowen Ni,Jun Yan,Yong Zhang,Xianchun Xia,Xinmin Chen,Zhonghu He.

[3]DEVELOPMENT AND CHARACTERIZATION OF 41 NOVEL EST-SSR MARKERS FOR PISUM SATIVUM (LEGUMINOSAE). Xu, Sheng-Chun,Gong, Ya-Ming,Hu, Qi-Zan,Zhang, Gu-Wen,Mao, Wei-Hua,Fu, Wan,Xian, Qiang-Qiang,Fu, Wan,Xian, Qiang-Qiang. 2012

[4]Identification and characterization of eighteen polymorphic microsatellite loci for a threatened freshwater fish, Botia superciliaris. Xiong, Fei,Liu, Hong-Yan,Duan, Xin-Bin,Chen, Da-Qing. 2015

[5]Microsatellite DNA Typing for Assessment of Genetic Variability in Taihu Goose: A Major Breed of China. Tang Qing-Ping,Zhang Shuang-Jie,Guo Jun,Chen Kuan-Wei,Lu Huo-Lin,Su Jian-Dong. 2009

[6]Isolation of novel microsatellite markers and their application for genetic diversity and parentage analyses in sika deer. Yang, Wanyun,Zheng, Junjun,Wei, Haijun,Wang, Guiwu,Yang, Fuhe,Jia, Boyin. 2018

[7]High Throughput Mining and Characterization of Microsatellites from Common Carp Genome. Ji, Peifeng,Zhang, Yan,Zhao, Zixia,Wang, Jian,Li, Jiongtang,Xu, Peng,Sun, Xiaowen,Li, Chao,Sun, Xiaowen. 2012

[8]First genetic linkage map for the mud crab (Scylla paramamosain) constructed using microsatellite and AFLP markers. Ma, H. Y.,Li, S. J.,Feng, N. N.,Ma, C. Y.,Wang, W.,Chen, W.,Ma, L. B.,Ma, H. Y.,Li, S. J.,Feng, N. N.,Ma, C. Y.,Wang, W.,Chen, W.,Ma, L. B.. 2016

[9]Diversity of Sicilian broccoli (Brassica oleracea var. italica) and cauliflower (Brassica oleracea var. botrytis) landraces and their distinctive bio-morphological, antioxidant, and genetic traits. Branca, Ferdinando,Chiarenza, Giuseppina Laura,Cavallaro, Chiara,Tribulato, Alessandro,Gu, Honghui,Zhao, Zhenqing. 2018

[10]Assessment of Genetic Diversity of Chinese Sand Pear Landraces (Pyrus pyrifolia Nakai) Using Simple Sequence Repeat Markers. Huang, Hongwen,Jiang, Zhengwang,Tang, Feiyan,Hu, Hongju,Chen, Qiliang. 2009

[11]SSR data mined from expressed sequence tags of phytoparasitic nematodes. Huang, Q. X.,Cheng, X. Y.,Xu, R. M.,Xie, B. Y.. 2010

[12]Population structure of Haemonchus contortus from seven geographical regions in China, determined on the basis of microsatellite markers. Yin, Fangyuan,Gasser, Robin B.,Li, Facai,Yang, Xin,Zhou, Yanqin,Zhao, Junlong,Fang, Rui,Hu, Min,Yin, Fangyuan,Li, Facai,Gasser, Robin B.,Bao, Min,Huang, Weiyi,Zou, Fengcai,Zhao, Guanghui,Wang, Chunren. 2016

[13]Population Dynamics of Phytophthora infestans in the Netherlands Reveals Expansion and Spread of Dominant Clonal Lineages and Virulence in Sexual Offspring. Li, Y.,van der Lee, T. A. J.,Evenhuis, A.,van den Bosch, G. B. M.,van Bekkum, P. J.,Forch, M. G.,van Gent-Pelzer, M. P. E.,van Raaij, H. M. G.,Kessel, G. J. T.,Li, Y.,Huang, S. W.,Jacobsen, E.,Vleeshouwers, V. G. A. A.,Govers, F.,Govers, F.. 2012

[14]A Set of Polymorphic Trinucleotide and Tetranucleotide Microsatellite Markers for Silver Crucian Carp (Carassius auratus gibelio) and Cross-Amplification in Crucian Carp. Zheng, X. H.,Lu, C. Y.,Zhao, Y. Y.,Lee, C.,Cao, D. C.,Chang, Y. M.,Liang, L. Q.,Sun, X. W.,Zheng, X. H.,Zhao, Y. Y.. 2010

[15]Polymorphic dinucleotide microsatellites in tongue sole (Cynoglossus semilaevis). Liao, X.,Shao, C. W.,Tian, Y. S.,Chen, S. L.. 2007

[16]Isolation and characterization of microsatellite markers from Pacific white shrimp (Litopenaeus vannamei). Jia Zhi-Ying,Sun Xiao-Wen,Liang Li-Qun,Li Da-Yu,Lei Qing-Quan. 2006

[17]Isolation and characterization of 10 polymorphic microsatellite loci from small yellow croaker (Pseudosciaena polyactis). Chen, Song-Lin,Xing, Shi-Chao,Xu, Gen-Bo,Liao, Xiaolin,Yang, Jing-Feng,Xing, Shi-Chao. 2009

[18]Development of microsatellite markers and genetic diversity analysis for Pelodiscus sinensis. Li, T.,Zhao, J.,Li, W.,Shi, Y.,Hong, X. Y.,Zhu, X. P.,Li, T.,Hong, X. Y.,Zhu, X. P.. 2016

[19]Genetic Structure and Eco-Geographical Differentiation of Cultivated Keng Rice (Oryza sativa L. subsp japonica) in China Revealed by Microsatellites. Zhang Dong-ling,Wang Mei-xing,Qi Yong-wen,Sun Jun-li,Wang Feng-mei,Li Jin-jie,Zhang Hong-liang,Li Zi-chao,Wang Mei-xing,Qi Yong-wen,Sun Jun-li. 2012

[20]Isolation and characterization of microsatellite markers from Fangzheng silver crucian carp, Carassius auratus gibelio (Bloch), and cross-amplification in the closely related species crucian carp, Carassius auratus auratus (Linnaeus). Jia, Zhi-Ying,Sun, Xiao-Wen,Liang, Li-Qun,Lu, Cui-Yun,Lei, Qing-Quan. 2006

作者其他论文 更多>>

微信小程序