RNA-dependent RNA polymerase 6 of rice (Oryza sativa) plays role in host defense against negative-strand RNA virus, Rice stripe virus

文献类型： 外文期刊

第一作者： Jiang, Lin

作者： Jiang, Lin;Qian, Dan;Zheng, Hong;Meng, Lin-Yan;Wei, Chun-Hong;Li, Yi;Chen, Jie;Le, Wen-Jing;Zhou, Tong;Zhou, Yi-Jun

作者机构：

关键词： RDR;Rice stripe virus;RNA silencing;Viral siRNA

期刊名称：VIRUS RESEARCH （ 影响因子：3.303； 五年影响因子：3.445 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： RNA-dependent RNA polymerases (RDRs) from fungi, plants and some invertebrate animals play fundamental roles in antiviral defense. Here, we investigated the role of RDR6 in the defense of economically important rice plants against a negative-strand RNA virus (Rice stripe virus, RSV) that causes enormous crop damage. In three independent transgenic lines (OsRDR6AS line A, B and C) in which OsRDR6 transcription levels were reduced by 70-80% through antisense silencing, the infection and disease symptoms of RSV were shown to be significantly enhanced. The hypersusceptibilities of the OsRDR6AS plants were attributed not to enhanced insect infestation but to enhanced virus infection. The rise in symptoms was associated with the increased accumulation of RSV genomic RNA in the OsRDR6AS plants. The deep sequencing data showed reduced RSV-derived siRNA accumulation in the OsRDR6AS plants compared with the wild type plants. This is the first report of the antiviral role of a RDR in a monocot crop plant in the defense against a negative-strand RNA virus and significantly expands upon the current knowledge of the antiviral roles of RDRs in the defense against different types of viral genomes in numerous groups of plants.

分类号： R37

  • 相关文献

[1]CMV2b-AGO Interaction Is Required for the Suppression of RDR-Dependent Antiviral Silencing in Arabidopsis. Fang, Yuan-Yuan,Zhao, Jian-Hua,Liu, Shang-Wu,Wang, Sheng,Duan, Cheng-Guo,Guo, Hui-Shan,Liu, Shang-Wu,Guo, Hui-Shan,Duan, Cheng-Guo. 2016

[2]Expressional and regulatory characterization of Arabidopsis RNA-dependent RNA polymerase 1. Xu, Tao,Zhang, Liang,Zhen, Jie,Fan, Yunliu,Zhang, Chunyi,Wang, Lei.

[3]Knock down of chitosanase expression in phytopathogenic fungus Fusarium solani and its effect on pathogenicity. Zhang, Bo,Li, Changsong,Liu, Huaiwei,Bao, Xiaoming. 2010

[4]Generation of double-virus-resistant marker-free transgenic potato plants. Bai, Yunfeng,Guo, Zhihua,Wang, Xiaoqi,Bai, Dongmei,Zhang, Weifeng. 2009

[5]Improved Pathogenicity of a Beet Black Scorch Virus Variant by Low Temperature and Co-infection with its Satellite RNA. Xu, Jin,Liu, Deshui,Zhang, Yongliang,Wang, Ying,Han, Chenggui,Li, Dawei,Yu, Jia-Lin,Wang, Xian-Bing,Xu, Jin. 2016

[6]Genome-wide identification of Dicer-like, Argonaute and RNA-dependent RNA polymerase gene families and their expression analyses in response to viral infection and abiotic stresses in Solanum lycopersicum. Mao, Zhen-Chuan,Kang, Hou-Xiang,Chen, Guo-Hua,Yang, Yu-Hong,Xie, Bing-Yan,Bai, Miao,Yang, Guo-Shun,Chen, Wen-Ting.

[7]Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Inhibits RNA-Mediated Gene Silencing by Targeting Ago-2. Chen, Jing,Zhang, Gaiping,Chen, Jing,Shi, Xibao,Wang, Li,Luo, Jun,Xing, Guangxu,Deng, Ruiguang,Zhang, Gaiping,Shi, Xibao,Zhang, Xiaozhuan,Yang, Hong,Li, Jinting,Wang, Aiping,Zhang, Gaiping. 2015

[8]High-Level Accumulation of Exogenous Small RNAs Not Affecting Endogenous Small RNA Biogenesis and Function in Plants. Shen Wan-xia,Zhou Chang-yong,Shen Wan-xia,Smith, Neil A.,Wang Ming-bo,Shen Wan-xia,Zhou Chang-yong. 2014

[9]SGS3 Cooperates with RDR6 in Triggering Geminivirus-Induced Gene Silencing and in Suppressing Geminivirus Infection in Nicotiana Benthamiana. Zhou, Xueping,Li, Fangfang,Wang, Yaqin,Zhou, Xueping. 2017

[10]Durable field resistance to wheat yellow mosaic virus in transgenic wheat containing the antisense virus polymerase gene. Chen, Ming,Ma, Youzhi,Du, Lipu,Ye, Xingguo,Pang, Junlan,Zhang, Xinmei,Li, Liancheng,Xu, Huijun,Sun, Liying,Chen, Jiong,Andika, Ida B.,Chen, Jianping.

[11]A novel strategy to enhance resistance to Cucumber mosaic virus in tomato by grafting to transgenic rootstocks. Bai Miao,Chen Wen-ting,Yang Gun-shun,Xie Bing-yan. 2016

[12]Silencing of NbXrn4 facilitates the systemic infection of Tobacco mosaic virus in Nicotiana benthamiana. Yan, Fei,Lu, Yuwen,Lin, Lin,Zheng, Hongying,Chen, Jianping,Yan, Fei,Lu, Yuwen,Lin, Lin,Zheng, Hongying,Chen, Jianping,Peng, Jiejun,Chen, Hairu,Yang, Jian,Jiang, Shanshan. 2011

[13]Characterization of small interfering RNAs derived from Rice black streaked dwarf virus in infected maize plants by deep sequencing. Li, Mingjun,Li, Yongqiang,Xia, Zihao,Zhou, Tao,Fan, Zaifeng,Li, Mingjun,Li, Yongqiang,Xia, Zihao,Zhou, Tao,Fan, Zaifeng,Di, Dianping,Zhang, Aihong,Miao, Hongqin.

[14]RNA-Dependent RNA Polymerase 1 from Nicotiana tabacum Suppresses RNA Silencing and Enhances Viral Infection in Nicotiana benthamiana. Ying, Xiao-Bao,Dong, Li,Zhu, Hui,Duan, Cheng-Guo,Du, Quan-Sheng,Lv, Dian-Qiu,Fang, Yuan-Yuan,Fang, Rong-Xiang,Guo, Hui-Shan,Ying, Xiao-Bao,Dong, Li,Zhu, Hui,Duan, Cheng-Guo,Du, Quan-Sheng,Lv, Dian-Qiu,Fang, Yuan-Yuan,Fang, Rong-Xiang,Guo, Hui-Shan,Ying, Xiao-Bao,Zhu, Hui,Lv, Dian-Qiu,Antonio Garcia, Juan.

[15]Resistance of non-transgenic papaya plants to papaya ringspot virus (PRSV) mediated by intron-containing hairpin dsRNAs expressed in bacteria. Shen, W.,Chen, Y.,Yan, P.,Tuo, D.,Li, X.,Zhou, P.,Shen, W.,Chen, Y.,Yan, P.,Tuo, D.,Li, X.,Zhou, P.,Yang, G..

[16]Production of transgenic rice new germplasm with strong resistance against two isolations of Rice stripe virus by RNA interference. Ma, Jin,Song, Yunzhi,Wu, Bin,Li, Kaidong,Zhu, Changxiang,Wen, Fujiang,Jiang, Mingsong. 2011

[17]Production of marker-free and RSV-resistant transgenic rice using a twin T-DNA system and RNAi. Jiang, Yayuan,Sun, Lin,Li, Kaidong,Song, Yunzhi,Zhu, Changxiang,Jiang, Mingsong.

[18]Virus resistance obtained in transgenic tobacco and rice by RNA interference using promoters with distinct activity. Zhang, C.,Song, Y.,Jiang, F.,Jiang, Y.,Zhu, C.,Wen, F.,Li, G..

[19]Dimeric artificial microRNAs mediate high resistance to RSV and RBSDV in transgenic rice plants. Sun, Lin,Lin, Chao,Du, Jinwen,Song, Yunzhi,Liu, Hongmei,Zhou, Shumei,Wen, Fujiang,Zhu, Changxiang,Jiang, Mingsong.

[20]Inheritance of resistance to rice stripe virus in rice line 'BL 1'. Ise, K,Ishikawa, K,Li, CY,Ye, CR. 2002

作者其他论文 更多>>

微信小程序