Computational identification of microRNAs in strawberry expressed sequence tags and validation of their precise sequences by miR-RACE

文献类型： 外文期刊

第一作者： Han, Jian

作者： Han, Jian;Yu, Hua-Ping;Wang, Chen;Liu, Hong;Fang, Jing-Gui;Dong, Qing-Hua;Ge, An-Jing;Zhao, Mi-Zhen

作者机构：

关键词： Fragaria vesca;microRNA;miR-RACE;qRT-PCR

期刊名称：JOURNAL OF HEREDITY （ 影响因子：2.645； 五年影响因子：3.069 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) are small, endogenously expressed, nonprotein-coding RNAs that regulate gene expression at the post-transcriptional level in both animals and plants through repressing translation or inducing mRNA degradation. A comprehensive strategy to identify new miRNA homologs by mining the repository of available strawberry expressed sequence tags (ESTs) was developed. By adopting a range of filtering criteria, we identified 11 potential miRNAs belonging to 5 miRNA families from 47890 Fragaria vesca EST sequences. Using 2 specific 5′ and 3′ miRNA RACE PCR reactions and a sequence-directed cloning method, we accurately determined both end sequences of 5 candidate miRNAs. Meanwhile, qRT-PCR was used to detect the expression of these 5 miRNAs in different strawberry organs and tissues at several growing stages. These newly identified F. vesca miRNAs (fve-miRNAs) and their expression information can improve our understanding of possible roles of fve-miRNAs in regulating the growth and development of F. vesca.

分类号： R394

  • 相关文献

[1]Characterization of the miR165 family and its target gene Pp-ATHB8 in Prunus persica. Zhang, Chunhua,Guo, Lei,Ma, Ruijuan,Yu, Mingliang,Zhang, Yanping,Han, Jian,Li, Xiaoying. 2012

[2]Identification of microRNAs from Amur grape (vitis amurensis Rupr.) by deep sequencing and analysis of microRNA variations with bioinformatics. Wang, Chen,Han, Jian,Kibet, Korir Nicholas,Kayesh, Emrul,Shangguan, Lingfei,Li, Xiaoying,Fang, Jinggui,Liu, Chonghuai. 2012

[3]Computational identification of microRNAs in peach expressed sequence tags and validation of their precise sequences by miR-RACE. Zhang, Yanping,Yu, Huaping,Han, Jian,Song, Changnian,Fang, Jinggui,Yu, Mingliang,Ma, Ruijuan.

[4]Bioinformatic identification and experimental validation of miRNAs from foxtail millet (Setaria italica). Han, Jun,Xie, Hao,Sun, Qingpeng,Lu, Min,Wang, Weixiang,Pan, Jinbao,Wang, Jun,Guo, Erhu.

[5]Mining, identification and function analysis of microRNAs and target genes in peanut (Arachis hypogaea L.). Zhang, Tingting,Hu, Shuhao,Yan, Caixia,Li, Chunjuan,Zhao, Xiaobo,Shan, Shihua,Hu, Shuhao,Wan, Shubo.

[6]Characterization and expression profiling of selected microRNAs in tomato (Solanum lycopersicon) 'Jiangshu14'. Korir, Nicholas Kibet,Li, Xiaoying,Xin, Sun,Wang, Chen,Changnian, Song,Kayesh, Emrul,Fang, Jinggui,Korir, Nicholas Kibet,Li, Xiaoying. 2013

[7]Profiling polyphenols of two diploid strawberry (Fragaria vesca) inbred lines using UHPLC-HRMSn. Sun, Jianghao,Chen, Pei,Liu, Xianjin,Yang, Tianbao,Slovin, Janet. 2014

[8]Genome-wide identification and comparative expression analysis of NBS-LRR-encoding genes upon Colletotrichum gloeosporioides infection in two ecotypes of Fragaria vesca. Li, Jing,Zhang, Qing-Yu,Duan, Ke,Ye, Zheng-Wen,Gao, Qing-Hua,Li, Jing,Gao, Zhi-Hong,Zhang, Qing-Yu,Wang, Fei.

[9]GhNAC12, a neutral candidate gene, leads to early aging in cotton (Gossypium hirsutum L). Fengli Zhao,JianhuiMa,Libei Li,Shuli Fan,Yaning Guo,Meizhen Song,Hengling Wei,Chaoyou Pang,Shuxun Yu. 2016

[10]Identification of active VQ motif-containing genes and the expression patterns under low nitrogen treatment in soybean. Wang, Xiaobo,Zhang, Haowei,Sun, Genlou,Jin, Yuan,Qiu, LiJuan,Qiu, LiJuan,Sun, Genlou.

[11]The development dynamics of the maize root transcriptome responsive to heavy metal Pb pollution. Gao, Jian,Zhang, Yongzhong,Lu, Chaolong,Peng, Hua,Shen, Yaou,Ding, Haiping,Zhang, Zhiming,Pan, Guangtang,Lin, Haijian,Peng, Hua,Luo, Mao,Li, Gaoke.

[12]Cucumis sativus L-type lectin receptor kinase (CsLecRK) gene family response to Phytophthora melonis, Phytophthora capsici and water immersion in disease resistant and susceptible cucumber cultivars. Wu, Tingquan,Wang, Rui,Xu, Xiaomei,He, Xiaoming,Sun, Baojuan,Zhong, Yujuan,Liang, Zhaojuan,Luo, Shaobo,Lin, Yu'e,Wu, Tingquan,Wang, Rui,Xu, Xiaomei,He, Xiaoming,Sun, Baojuan,Zhong, Yujuan,Liang, Zhaojuan,Luo, Shaobo.

[13]Transcriptome comparison of resistant and susceptible sesame (Sesamum indicum L.) varieties inoculated with Fusarium oxysporum f.sp.sesami. Wei, Libin,Zhang, Haiyang,Duan, Yinghui,Li, Chun,Chang, Shuxian,Miao, Hongmei.

[14]Use of Digital Gene Expression to Discriminate Gene Expression in Different Developmental Stages of Rosa chinensis 'Pallida'. Yan, H. J.,Zhang, H.,Li, S. B.,Zhang, T.,Wang, Q. G.,Zhou, N. N.,Chen, M.,Tang, K. X.,Yang, J. K..

[15]Identification of a germ cell marker gene, the dead end homologue, in Chinese sturgeon Acipenser sinensis. Yang Xiaoge,Ye Huan,Wei Qiwei,Yang Xiaoge,Yue Huamei,Ye Huan,Li Chuangju,Wei Qiwei,Yue Huamei,Li Chuangju,Wei Qiwei.

[16]Identification of suitable reference genes for quantitative real-time PCR normalization in blotched snakehead Channa maculata. Mao, H.,Chen, K.,Zhu, X.,Luo, Q.,Zhao, J.,Li, W.,Wu, X.,Xu, H.,Mao, H.,Chen, K.,Wu, X.,Xu, H..

[17]Expression Differences of Resistance-Related Genes Induced by Cycloxaprid Using qRT-PCR in the Female Adult of Sogatella furcifera (Hemiptera: Delphacidae). Jin, Jian-Xue,Jin, Dao-Chao,Jin, Jian-Xue,Li, Feng-Liang,Cheng, Ying,Li, Wen-Hong,Ye, Zhao-Chun,Zhou, Yu-Hang.

[18]Characterization of miRNAs responsive to exogenous ethylene in grapevine berries at whole genome level. Zhao, Fanggui,Wang, Chen,Han, Jian,Zhu, Xudong,Li, Xiaopeng,Fang, Jinggui,Zhao, Fanggui,Wang, Xicheng.

[19]Evaluation of appropriate reference genes for gene expression studies in pepper by quantitative real-time PCR. Bin, Wang Shu,Wei, Liu Ke,Ping, Diao Wei,Li, Zhi,Wei, Ge,Bing, Liu Jin,Gui, Pan Bao,Jian, Wan Hong,Feng, Chen Jin,Wei, Liu Ke.

[20]Genome-wide analysis of terpene synthases in soybean: Functional characterization of GmTPS3. Huang, Fang,Wang, Xia,Wang, Jiao,Yu, Deyue,Liu, Jianyu,Zhang, Man,Zheng, Rui.

作者其他论文 更多>>

微信小程序