Development of an immunochromatographic strip for rapid detection of antibodies against classical swine fever virus
文献类型: 外文期刊
第一作者: Li, Xuewu
作者: Li, Xuewu;Wang, Li;Shi, Xibao;Zhao, Dong;Yang, Jifei;Yang, Suzhen;Zhang, Gaiping
作者机构:
关键词: Classical swine fever virus;Immunochromatographic strip;Chimeric protein;Rapid antibody detection
期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )
ISSN:
年卷期:
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收录情况: SCI
摘要: The genes encoding the Erns and E2 antigen epitopes of classical swine fever virus (CSFV) were expressed as a chimeric protein in Escherichia coli BL21 by pET expression system. The antigenicity of the expressed protein CnC2 was identified by indirect enzyme-linked immunoabsorbant assay (ELISA) and immunoblot with anti-CSFV antibodies. Based on the CnC2 protein, an immunochromatographic strip was developed to evaluate the antibody titer of serum samples from swine vaccinated with CSFV vaccine rapidly. The chimeric protein used as a detector was labeled with colloidal gold. Staphylococcal protein A (SPA) and anti-CnC2 monoclonal antibodies (mAbs) were blotted onto the nitrocellulose membrane as the test and control lines, respectively. The strip assay could be performed within 5 min, which did not require any special equipment or skills. Through testing sera against various strains of CSFV, the sensitivity of the strip was determined to be 97.0% (65/67) and the specificity was 100% (98/98). The strip results were consistent with those of the existing commercial ELISA kit, and their correlation coefficient was 0.935. In conclusion, the immunochromatographic strip was an acceptable method for surveying CSFV-antibody titers in pigs. (C) 2011 Elsevier B.V. All rights reserved.
分类号: R37
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