Reverse transcription loop-mediated isothermal amplification of RNA for sensitive and rapid detection of southern rice black-streaked dwarf virus
文献类型: 外文期刊
第一作者: Zhou, Tong
作者: Zhou, Tong;Du, Linlin;Fan, Yongjian;Zhou, Yijun;Zhou, Tong;Du, Linlin;Zhou, Yijun
作者机构:
关键词: SRBSDV;RT-LAMP;Detection
期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Southern rice black-streaked dwarf virus (SRBSDV) causes one of the most serious viral diseases of rice in Southeast Asia. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of SRBSDV using total RNA extracted from rice tissues and the insect pest, white-backed planthopper. The assay was based on a set of four primers matching a total of six sequences in the 59 region of SRBSDV genome. Presence of the virus could be detected in RT-LAMP reactions containing 1.2 x 10(-6) mu g of a total RNA extract, which was ten times more sensitive than a classical RT-PCR assay. The SRBSDV could be distinguished from the closely related rice black-streaked dwarf virus (RBSDV) by this method, indicating a high degree of specificity. This simple and sensitive RT-LAMP assay shows potential for detection of SRBSDV in field samples of hosts or vectors at a relatively low cost. (C) 2011 Elsevier B.V. All rights reserved.
分类号: R37
- 相关文献
作者其他论文 更多>>
-
Investigation and characterization of rice dwarfing epidemic caused by southern rice black-streaked dwarf virus in Jiangsu in 2023
作者:Li, Chenyang;Yang, Wenxuan;Du, Linlin;Lin, Feng;Lan, Ying;Zhou, Tong;Yang, Wenxuan;Zhang, Yuanming;Qiu, Yanglin;Xu, Kai;Zhou, Tong;Zhu, Feng
关键词:Rice viral disease; SRBSDV; Jiangsu rice dwarfing epidemic; Virus detection; Koch's postulates
-
Development of a transgenic rice line with strong and broad resistance against four devastating rice viruses through expressing a single hairpin RNA construct
作者:Li, Chenyang;Wu, Jianxiang;Fu, Shuai;Xu, Yi;Wang, Yaqin;Zhou, Xueping;Li, Chenyang;Lan, Ying;Lin, Feng;Du, Linlin;Zhou, Tong;Yang, Xiuling;Zhou, Xueping
关键词:rice; resistance; virus; RNA interference; hairpin RNA transgene
-
Transgenic expression of artificial microRNA targeting soybean mosaic virus P1 gene confers virus resistance in plant
作者:Latif, Muhammad Faizan;Tan, Jingquan;Zhang, Wang;Yang, Wenxuan;Zhuang, Tinghui;Lu, Wenlong;Qiu, Yanglin;Du, Xinying;Zhuang, Xinjian;Zhou, Tong;Yin, Jinlong;Xu, Kai;Zhou, Tong;Kundu, Jiban K.;Kundu, Jiban K.
关键词:MicroRNA; Potyvirus; SMV; P1 gene; Transgenic; Virus resistance
-
Alleles on locus chromosome 4B from different parents confer tiller number and the yield-associated traits in wheat
作者:Li, Yahui;Zhou, Yijun;Li, Yahui;Hu, Jinghuang;Zhou, Yang;Zhang, Hongjun;Yang, Li;Liu, Hongwei;Li, Hongjie;Qiu, Dan;Wu, Qiuhong;Liu, Zhiyong;Qu, Yunfeng;Lin, Huailong;Du, Jiuyuan;Hou, Lu;Ma, Lin
关键词:Triticum aestivum; Quantitative trait loci; Tiller number; Spike traits; Grain traits
-
The miR-184-3p promotes rice black-streaked dwarf virus infection by suppressing Ken in Laodelphax striatellus (Fallén)
作者:Wu, Wei;Wang, Man;Deng, Zhiting;Xi, Minmin;Dong, Yan;Wang, Haitao;Zhang, Jianhua;Zhou, Yijun;Xu, Qiufang;Wu, Wei;Deng, Zhiting;Xi, Minmin;Wang, Changchun;Xu, Qiufang
关键词:miR-184-3p; Laodelphax striatellus; rice black-streaked dwarf virus; Ken; virus infection
-
Plasmodesmata-associated Flotillin positively regulates broad-spectrum virus cell-to-cell trafficking
作者:Ge, Panpan;Lu, Hong;Wang, Wei;Ma, Yonghuan;Cui, Feng;Ge, Panpan;Ma, Yonghuan;Cui, Feng;Li, Yi;Zhou, Tong;Wei, Taiyun;Wu, Jianguo
关键词:Flotillin; plasmodesmata-associated protein; rice; rice stripe virus; southern rice black-streaked dwarf virus; rice dwarf virus
-
Role of Sox3 in Estradiol-Induced Sex Reversal in Pelodiscus sinensis
作者:Zhou, Tong;Cao, Jizeng;Chen, Guobin;Wang, Yubin;Zou, Guiwei;Liang, Hongwei;Cao, Jizeng
关键词:gonadal differentiation; Pelodiscus sinensis; Sox3; RNAi; sex reversal