Reverse transcription loop-mediated isothermal amplification of RNA for sensitive and rapid detection of southern rice black-streaked dwarf virus

文献类型: 外文期刊

第一作者: Zhou, Tong

作者: Zhou, Tong;Du, Linlin;Fan, Yongjian;Zhou, Yijun;Zhou, Tong;Du, Linlin;Zhou, Yijun

作者机构:

关键词: SRBSDV;RT-LAMP;Detection

期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )

ISSN:

年卷期:

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收录情况: SCI

摘要: Southern rice black-streaked dwarf virus (SRBSDV) causes one of the most serious viral diseases of rice in Southeast Asia. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of SRBSDV using total RNA extracted from rice tissues and the insect pest, white-backed planthopper. The assay was based on a set of four primers matching a total of six sequences in the 59 region of SRBSDV genome. Presence of the virus could be detected in RT-LAMP reactions containing 1.2 x 10(-6) mu g of a total RNA extract, which was ten times more sensitive than a classical RT-PCR assay. The SRBSDV could be distinguished from the closely related rice black-streaked dwarf virus (RBSDV) by this method, indicating a high degree of specificity. This simple and sensitive RT-LAMP assay shows potential for detection of SRBSDV in field samples of hosts or vectors at a relatively low cost. (C) 2011 Elsevier B.V. All rights reserved.

分类号: R37

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